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MESG *Phosphate assay reagent*
In the presence of inorganic phosphate MESG is converted to 2-amino-6-mercapto-7-methlpurine by purine nucleoside phosphorylase (EC 2.4.2.1) with absorption wavelength shift to red. This feature has been used to quantifying phosphate spectrophotometrically. We offer a convenient MESG-based phosphate assay kit (#21659).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of MESG *Phosphate assay reagent* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 319.142 µL | 1.596 mL | 3.191 mL | 15.957 mL | 31.914 mL |
| 5 mM | 63.828 µL | 319.142 µL | 638.284 µL | 3.191 mL | 6.383 mL |
| 10 mM | 31.914 µL | 159.571 µL | 319.142 µL | 1.596 mL | 3.191 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Citations
View all 3 citations: Citation Explorer
Polyphosphate Coated Nanoparticles: Enzyme-Activated Charge-Reversal Gene Delivery Systems
Authors: Akku{\c{s}}-Da{\u{g}}deviren, Zeynep Burcu and Ar{\i}soy, Sema and Friedl, Julian David and F{\"u}rst, Andrea and Saleh, Ahmad and Bernkop-Schn{\"u}rch, Andreas
Journal: International Journal of Pharmaceutics (2023): 123474
Authors: Akku{\c{s}}-Da{\u{g}}deviren, Zeynep Burcu and Ar{\i}soy, Sema and Friedl, Julian David and F{\"u}rst, Andrea and Saleh, Ahmad and Bernkop-Schn{\"u}rch, Andreas
Journal: International Journal of Pharmaceutics (2023): 123474
Sacrificial crystal templating of hyaluronic acid-based hydrogels
Authors: Thomas, Richelle C and Chung, Paul E and Modi, Shan P and Hardy, John G and Schmidt, Christine E
Journal: European Polymer Journal (2016)
Authors: Thomas, Richelle C and Chung, Paul E and Modi, Shan P and Hardy, John G and Schmidt, Christine E
Journal: European Polymer Journal (2016)
Osteogenic cell cultures cannot utilize exogenous sources of synthetic polyphosphate for mineralization
Authors: Ariganello, Marianne B and Omelon, Sidney and Variola, Fabio and Wazen, Rima M and Moffatt, Pierre and Nanci, Antonio
Journal: Journal of cellular biochemistry (2014): 2089--2102
Authors: Ariganello, Marianne B and Omelon, Sidney and Variola, Fabio and Wazen, Rima M and Moffatt, Pierre and Nanci, Antonio
Journal: Journal of cellular biochemistry (2014): 2089--2102
References
View all 8 references: Citation Explorer
Relationship between activating and editing functions of the adenylation domain of apo-tyrocidin synthetase 1 (apo-TY1)
Authors: Bucevic-Popovic V, Pavela-Vrancic M, Dieckmann R, Von Dohren H.
Journal: Biochimie (2006): 265
Authors: Bucevic-Popovic V, Pavela-Vrancic M, Dieckmann R, Von Dohren H.
Journal: Biochimie (2006): 265
Purine nucleoside phosphorylase activity in rat cerebrospinal fluid
Authors: Silva RG, Santos DS, Basso LA, Oses JP, Wofchuk S, Portela LV, Souza DO.
Journal: Neurochem Res (2004): 1831
Authors: Silva RG, Santos DS, Basso LA, Oses JP, Wofchuk S, Portela LV, Souza DO.
Journal: Neurochem Res (2004): 1831
Characterization of the interactions between the small GTPase RhoA and its guanine nucleotide exchange factors
Authors: Tan YC, Wu H, Wang WN, Zheng Y, Wang ZX.
Journal: Anal Biochem (2002): 156
Authors: Tan YC, Wu H, Wang WN, Zheng Y, Wang ZX.
Journal: Anal Biochem (2002): 156
A continuous spectrophotometric assay for aspartate transcarbamylase and ATPases
Authors: Rieger CE, Lee J, Turnbull JL.
Journal: Anal Biochem (1997): 86
Authors: Rieger CE, Lee J, Turnbull JL.
Journal: Anal Biochem (1997): 86
Continuous monitoring of Pi release following nucleotide hydrolysis in actin or tubulin assembly using 2-amino-6-mercapto-7-methylpurine ribonucleoside and purine-nucleoside phosphorylase as an enzyme-linked assay
Authors: Melki R, Fievez S, Carlier MF.
Journal: Biochemistry (1996): 12038
Authors: Melki R, Fievez S, Carlier MF.
Journal: Biochemistry (1996): 12038
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