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MeO-Succ-Arg-Pro-Tyr-AMC
MeO-Succ-Arg-Pro-Tyr-AMC is a sensitive fluorogenic substrate for chymotrypsin-like proteases. The non-fluorescent substrate generates a bright blue fluorescent AMC product that has an emission spectra that can be easily detected with the DAPI filter set. It has been used for monitoring the protease activity of stratum corneum chymotryptic enzyme (SCCE).
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of MeO-Succ-Arg-Pro-Tyr-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 121.984 µL | 609.92 µL | 1.22 mL | 6.099 mL | 12.198 mL |
| 5 mM | 24.397 µL | 121.984 µL | 243.968 µL | 1.22 mL | 2.44 mL |
| 10 mM | 12.198 µL | 60.992 µL | 121.984 µL | 609.92 µL | 1.22 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Citations
View all 1 citations: Citation Explorer
Carbon dioxide ameliorates reduced desquamation in dry scaly skin via protease activation
Authors: Fukagawa, Satoko and Takahashi, Ayami and Sayama, Keimon and Mori, Shinobu and Murase, Takatoshi
Journal: International Journal of Cosmetic Science (2020)
Authors: Fukagawa, Satoko and Takahashi, Ayami and Sayama, Keimon and Mori, Shinobu and Murase, Takatoshi
Journal: International Journal of Cosmetic Science (2020)
References
View all 101 references: Citation Explorer
A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease
Authors: Bodenreider C, Beer D, Keller TH, Sonntag S, Wen D, Yap L, Yau YH, Shochat SG, Huang D, Zhou T, Caflisch A, Su XC, Ozawa K, Otting G, Vasudevan SG, Lescar J, Lim SP.
Journal: Anal Biochem (2009): 195
Authors: Bodenreider C, Beer D, Keller TH, Sonntag S, Wen D, Yap L, Yau YH, Shochat SG, Huang D, Zhou T, Caflisch A, Su XC, Ozawa K, Otting G, Vasudevan SG, Lescar J, Lim SP.
Journal: Anal Biochem (2009): 195
Prokaryotic expression and Identification of dual-fluorescence fusion proteins of small ubiquitin-like modifier and sentrin-specific protease
Authors: Wang X, Guo J, Wang J, Li S, Sun L, Lu J.
Journal: Sheng Wu Gong Cheng Xue Bao (2009): 701
Authors: Wang X, Guo J, Wang J, Li S, Sun L, Lu J.
Journal: Sheng Wu Gong Cheng Xue Bao (2009): 701
Real-time monitoring of human enterovirus (HEV)-infected cells and anti-HEV 3C protease potency by fluorescence resonance energy transfer
Authors: Tsai MT, Cheng YH, Liu YN, Liao NC, Lu WW, Kung SH.
Journal: Antimicrob Agents Chemother (2009): 748
Authors: Tsai MT, Cheng YH, Liu YN, Liao NC, Lu WW, Kung SH.
Journal: Antimicrob Agents Chemother (2009): 748
Dual-function probe to detect protease activity for fluorescence measurement and 19F MRI
Authors: Mizukami S, Takikawa R, Sugihara F, Shirakawa M, Kikuchi K.
Journal: Angew Chem Int Ed Engl (2009): 3641
Authors: Mizukami S, Takikawa R, Sugihara F, Shirakawa M, Kikuchi K.
Journal: Angew Chem Int Ed Engl (2009): 3641
Real-time detection of caspase-3-like protease activation in vivo using fluorescence resonance energy transfer during plant programmed cell death induced by ultraviolet C overexposure
Authors: Zhang L, Xu Q, Xing D, Gao C, Xiong H.
Journal: Plant Physiol (2009): 1773
Authors: Zhang L, Xu Q, Xing D, Gao C, Xiong H.
Journal: Plant Physiol (2009): 1773
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