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MCA succinimidyl ester [7-Methoxycoumarin-4-acetic acid, succinimidyl ester]
7-Methoxycoumarin-4-acetic acid (MCA) is widely used for developing FRET peptide substrates for analyzing protease activities. It is commonly paired with dinitrophenyl (DNP) group. It is also used for HPLC derivatization by fluorescence detection. MCA succinimidyl ester selectively reacts with aliphatic amines.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of MCA succinimidyl ester [7-Methoxycoumarin-4-acetic acid, succinimidyl ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 301.859 µL | 1.509 mL | 3.019 mL | 15.093 mL | 30.186 mL |
| 5 mM | 60.372 µL | 301.859 µL | 603.719 µL | 3.019 mL | 6.037 mL |
| 10 mM | 30.186 µL | 150.93 µL | 301.859 µL | 1.509 mL | 3.019 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
References
View all 6 references: Citation Explorer
Fluorescent labeling of peptides on solid phase
Authors: Katritzky AR, Yoshioka M, Narindoshvili T, Chung A, Johnson JV.
Journal: Org Biomol Chem (2008): 4582
Authors: Katritzky AR, Yoshioka M, Narindoshvili T, Chung A, Johnson JV.
Journal: Org Biomol Chem (2008): 4582
Development of sensitive cathepsin G fluorogenic substrate using combinatorial chemistry methods
Authors: Lesner A, Wysocka M, Guzow K, Wiczk W, Legowska A, Rolka K.
Journal: Anal Biochem (2008): 306
Authors: Lesner A, Wysocka M, Guzow K, Wiczk W, Legowska A, Rolka K.
Journal: Anal Biochem (2008): 306
Preparation and characterization of two LysB29 specifically labelled fluorescent derivatives of human insulin
Authors: Ciencialova A, Zakova L, Jiracek J, Barthova J, Barth T.
Journal: J Pept Sci (2004): 470
Authors: Ciencialova A, Zakova L, Jiracek J, Barthova J, Barth T.
Journal: J Pept Sci (2004): 470
Substrate binding of gelatinase B induces its enzymatic activity in the presence of intact propeptide
Authors: Bannikov GA, Karelina TV, Collier IE, Marmer BL, Goldberg GI.
Journal: J Biol Chem (2002): 16022
Authors: Bannikov GA, Karelina TV, Collier IE, Marmer BL, Goldberg GI.
Journal: J Biol Chem (2002): 16022
Analysis of 5-fluorouracil in plasma by precolumn derivatization with 4-bromomethyl-7-methoxycoumarin, followed by multi-dimensional high-performance liquid chromatography
Authors: Kindberg CG, Riley CM, Stobaugh JF, Slavik M.
Journal: J Chromatogr (1989): 431
Authors: Kindberg CG, Riley CM, Stobaugh JF, Slavik M.
Journal: J Chromatogr (1989): 431
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