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mBCl [Monochlorobimane] *CAS 76421-73-3*
Monochloromobimane (mBCl) is essentially nonfluorescent until reacted with a thiol group. It readily reacts with several low molecular weight thiols, including cysteine, glutathione, N-acetylcysteine, mercaptopurine, peptides and plasma thiols. The glutathione conjugate of mBCl has absorption/emission maxima ~394/490 nm.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of mBCl [Monochlorobimane] *CAS 76421-73-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 441.189 µL | 2.206 mL | 4.412 mL | 22.059 mL | 44.119 mL |
| 5 mM | 88.238 µL | 441.189 µL | 882.379 µL | 4.412 mL | 8.824 mL |
| 10 mM | 44.119 µL | 220.595 µL | 441.189 µL | 2.206 mL | 4.412 mL |
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Citations
View all 1 citations: Citation Explorer
Combined structural and functional imaging of the kidney reveals major axial differences in proximal tubule endocytosis
Authors: Schuh, Claus D and Polesel, Marcello and Platonova, Evgenia and Haenni, Dominik and Gassama, Alkaly and Tokonami, Natsuko and Ghazi, Susan and Bugarski, Milica and Devuyst, Olivier and Ziegler, Urs and others,
Journal: Journal of the American Society of Nephrology (2018): 2696--2712
Authors: Schuh, Claus D and Polesel, Marcello and Platonova, Evgenia and Haenni, Dominik and Gassama, Alkaly and Tokonami, Natsuko and Ghazi, Susan and Bugarski, Milica and Devuyst, Olivier and Ziegler, Urs and others,
Journal: Journal of the American Society of Nephrology (2018): 2696--2712
References
View all 19 references: Citation Explorer
Use of flow cytometry and monochlorobimane to quantitate intracellular glutathione concentrations in feline leukocytes
Authors: Webb C, Bedwell C, Guth A, Avery P, Dow S.
Journal: Vet Immunol Immunopathol (2006): 129
Authors: Webb C, Bedwell C, Guth A, Avery P, Dow S.
Journal: Vet Immunol Immunopathol (2006): 129
Formation and rapid export of the monochlorobimane-glutathione conjugate in cultured rat astrocytes
Authors: Waak J, Dringen R.
Journal: Neurochem Res (2006): 1409
Authors: Waak J, Dringen R.
Journal: Neurochem Res (2006): 1409
Effects of monochlorobimane on cerebral ischemia-induced damage to mitochondria
Authors: Oshikawa S, Miyake-Takagi K, Takagi N, Abe T, Kuruhara Y, Takeo S.
Journal: Biol Pharm Bull (2004): 166
Authors: Oshikawa S, Miyake-Takagi K, Takagi N, Abe T, Kuruhara Y, Takeo S.
Journal: Biol Pharm Bull (2004): 166
Measurement of the intracellular distribution of reduced glutathione in cultured rat hepatocytes using monochlorobimane and confocal laser scanning microscopy
Authors: Stevenson D, Wokosin D, Girkin J, Grant MH.
Journal: Toxicol In Vitro (2002): 609
Authors: Stevenson D, Wokosin D, Girkin J, Grant MH.
Journal: Toxicol In Vitro (2002): 609
Fluorometric microplate assay to measure glutathione S-transferase activity in insects and mites using monochlorobimane
Authors: Nauen R, Stumpf N.
Journal: Anal Biochem (2002): 194
Authors: Nauen R, Stumpf N.
Journal: Anal Biochem (2002): 194
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