MagaDye™ 588-ddTTP

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	~1900
Solvent	Water

Spectral properties

Absorbance (nm)	498
Excitation (nm)	498
Emission (nm)	588

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

~1900

Absorbance (nm)

498

Excitation (nm)

498

Emission (nm)

588

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase. It was developed by Frederick Sanger and colleagues in 1977. Although the newer NGS technologies are becoming common in clinical research labs due to their higher throughput capabilities and lower costs per sample, Sanger sequencing with 99.99% accuracy is still the “gold standard” for clinical research sequencing. The four distinct fluorescent ddNTPs (labeled with BigDye®, BigDye® is the trademark of ThermoFisher) are the critical components for performing Sanger sequencing. MagaDye™ 588-ddTTP is equivalent to BigDye dTMR color with almost identical spectra.

Spectrum

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Spectral properties

Absorbance (nm)	498
Excitation (nm)	498
Emission (nm)	588

Images

Figure 1. Chemical structure for MagaDye™ 588-ddTTP.

References

View all 50 references: Citation Explorer

Update on molecular companion diagnostics - a future in personalized medicine beyond Sanger sequencing.
Authors: Campbell, Michelle Renee
Journal: Expert review of molecular diagnostics (2020)

Rapid, Inexpensive Measurement of Synthetic Bacterial Community Composition by Sanger Sequencing of Amplicon Mixtures.
Authors: Cermak, Nathan and Datta, Manoshi Sen and Conwill, Arolyn
Journal: iScience (2020): 100915

Guidelines for Sanger sequencing and molecular assay monitoring.
Authors: Crossley, Beate M and Bai, Jianfa and Glaser, Amy and Maes, Roger and Porter, Elizabeth and Killian, Mary Lea and Clement, Travis and Toohey-Kurth, Kathy
Journal: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagn (2020): 1040638720905833

A novel gross deletion and breakpoint junction sequence analysis of ATP7B in a Chinese family with Wilson disease using next‑generation sequencing and Sanger sequencing.
Authors: Liu, Wei-Liang and Li, Fang and Liu, Lu and Chen, Wei and He, Zhi-Xu and Gu, Hao and Ai, Rong
Journal: Molecular medicine reports (2020): 517-523

Concurrent Cultivation of Mycobacterium avium and Mycobacterium intracellulare Identified by a Single Sanger Sequencing of the 16S Gene.
Authors: Han, Xiang Y and Golshan, Mohammad A and Bowman, Christopher J
Journal: Journal of clinical microbiology (2020)

Shall I trust the report? Variable performance of Sanger sequencing revealed by deep sequencing on HIV drug resistance mutation detection.
Authors: Chen, Nan-Yu and Kao, Shu-Wei and Liu, Zhuo-Hao and Wu, Ting-Shu and Tsai, Chia-Lung and Lin, Hsi-Hsun and Wong, Wing-Wai and Chang, Yea-Yuan and Chen, Shu-Sheng and Ku, Stephane Wen-Wei
Journal: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases (2020): 182-191

Detection of TERT promoter mutation in serum cell-free DNA using wild-type blocking PCR combined with Sanger sequencing in hepatocellular carcinoma.
Authors: Akuta, Norio and Suzuki, Fumitaka and Kobayashi, Mariko and Fujiyama, Shunichiro and Kawamura, Yusuke and Sezaki, Hitomi and Hosaka, Tetsuya and Kobayashi, Masahiro and Saitoh, Satoshi and Arase, Yasuji and Ikeda, Kenji and Suzuki, Yoshiyuki and Kumada, Hiromitsu
Journal: Journal of medical virology (2020)

Insights into pituitary tumorigenesis: from Sanger sequencing to next-generation sequencing and beyond.
Authors: Shen, Angeline Jia Jia and King, James and Scott, Hamish and Colman, Peter and Yates, Christopher J
Journal: Expert review of endocrinology & metabolism (2019): 399-418

[Diagnostic value of multiplex ligation dependent probe amplification combined with Sanger sequencing in 21-hydroxylase deficiency].
Authors: Gao, Y J and Yu, B Q and Lu, L and Tong, A L and Chen, S and Mao, J F and Wang, X and Wu, X Y and Nie, M
Journal: Zhonghua yi xue za zhi (2019): 432-437

Justification of direct Sanger sequencing application for detection of KIT and PDGFRα gene mutations in formalin-fixed, paraffin-embedded samples from gastrointestinal stromal tumours.
Authors: Kiwerska, Katarzyna and Wroblewska, Joanna and Kaluzna, Apolonia and Marszalek, Andrzej
Journal: Journal of clinical pathology (2019)