MagaDye™ 588-ddTTP

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase. It was developed by Frederick Sanger and colleagues in 1977. Although the newer NGS technologies are becoming common in clinical research labs due to their higher throughput capabilities and lower costs per sample, Sanger sequencing with 99.99% accuracy is still the “gold standard” for clinical research sequencing. The four distinct fluorescent ddNTPs (labeled with BigDye®, BigDye® is the trademark of ThermoFisher) are the critical components for performing Sanger sequencing. MagaDye™ 588-ddTTP is equivalent to BigDye dTMR color with almost identical spectra.

Spectrum

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References

View all 50 references: Citation Explorer

Update on molecular companion diagnostics - a future in personalized medicine beyond Sanger sequencing.
Authors: Campbell, Michelle Renee
Journal: Expert review of molecular diagnostics (2020)

Rapid, Inexpensive Measurement of Synthetic Bacterial Community Composition by Sanger Sequencing of Amplicon Mixtures.
Authors: Cermak, Nathan and Datta, Manoshi Sen and Conwill, Arolyn
Journal: iScience (2020): 100915

Guidelines for Sanger sequencing and molecular assay monitoring.
Authors: Crossley, Beate M and Bai, Jianfa and Glaser, Amy and Maes, Roger and Porter, Elizabeth and Killian, Mary Lea and Clement, Travis and Toohey-Kurth, Kathy
Journal: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagn (2020): 1040638720905833

A novel gross deletion and breakpoint junction sequence analysis of ATP7B in a Chinese family with Wilson disease using next‑generation sequencing and Sanger sequencing.
Authors: Liu, Wei-Liang and Li, Fang and Liu, Lu and Chen, Wei and He, Zhi-Xu and Gu, Hao and Ai, Rong
Journal: Molecular medicine reports (2020): 517-523

Concurrent Cultivation of Mycobacterium avium and Mycobacterium intracellulare Identified by a Single Sanger Sequencing of the 16S Gene.
Authors: Han, Xiang Y and Golshan, Mohammad A and Bowman, Christopher J
Journal: Journal of clinical microbiology (2020)

Page updated on December 2, 2024

Ordering information

Price
Unit size	5 nmoles 50 nmoles
Catalog Number	1706117065
Quantity

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Additional ordering information

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Physical properties

Molecular weight	~1900
Solvent	Water

Spectral properties

Absorbance (nm)	498
Excitation (nm)	498
Emission (nm)	588

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501