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Mag-Fura-2 tetrapotassium salt *Cell impermeant*
Mag-Fura-2, tetrapotassium salt is an intracellular magnesium indicator that is ratiometric and UV light-excitable. This water-soluble salt form is useful for intracellular loading by microinjection or infusion from patch pipette. This particular magnesium indicator is also used for high concentration calcium detection.
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Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Mag-Fura-2 tetrapotassium salt *Cell impermeant* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 170.451 µL | 852.253 µL | 1.705 mL | 8.523 mL | 17.045 mL |
| 5 mM | 34.09 µL | 170.451 µL | 340.901 µL | 1.705 mL | 3.409 mL |
| 10 mM | 17.045 µL | 85.225 µL | 170.451 µL | 852.253 µL | 1.705 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
References
View all 15 references: Citation Explorer
Tuning the Spectroscopic Properties of Ratiometric Fluorescent Metal Indicators: Experimental and Computational Studies on Mag-Fura-2 and Analogues
Authors: Zhang G, Jacquemin D, Buccella D.
Journal: J Phys Chem B. (2017)
Authors: Zhang G, Jacquemin D, Buccella D.
Journal: J Phys Chem B. (2017)
Fluorescence measurements of free [Mg2+] by use of mag-fura 2 in Salmonella enterica
Authors: Froschauer EM, Kolisek M, Dieterich F, Schweigel M, Schweyen RJ.
Journal: FEMS Microbiol Lett (2004): 49
Authors: Froschauer EM, Kolisek M, Dieterich F, Schweigel M, Schweyen RJ.
Journal: FEMS Microbiol Lett (2004): 49
Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC
Authors: Hyrc KL, Bownik JM, Goldberg MP.
Journal: Cell Calcium (2000): 75
Authors: Hyrc KL, Bownik JM, Goldberg MP.
Journal: Cell Calcium (2000): 75
Mag-Fura-2 (Furaptra) exhibits both low (microM) and high (nM) affinity for Ca2+
Authors: Martinez-Zaguilan R, Parnami J, Martinez GM.
Journal: Cell Physiol Biochem (1998): 158
Authors: Martinez-Zaguilan R, Parnami J, Martinez GM.
Journal: Cell Physiol Biochem (1998): 158
Intracellular Mg2+ regulation in voltage-clamped Helix aspersa neurones measured with mag-fura-2 and Mg(2+)-sensitive microelectrodes
Authors: Kennedy HJ., undefined
Journal: Exp Physiol (1998): 449
Authors: Kennedy HJ., undefined
Journal: Exp Physiol (1998): 449
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