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Mag-Fura-2 tetrapotassium salt *Cell impermeant*

Mag-Fura-2, tetrapotassium salt is an intracellular magnesium indicator that is ratiometric and UV light-excitable. This water-soluble salt form is useful for intracellular loading by microinjection or infusion from patch pipette. This particular magnesium indicator is also used for high concentration calcium detection.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Mag-Fura-2 tetrapotassium salt *Cell impermeant* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM170.451 µL852.253 µL1.705 mL8.523 mL17.045 mL
5 mM34.09 µL170.451 µL340.901 µL1.705 mL3.409 mL
10 mM17.045 µL85.225 µL170.451 µL852.253 µL1.705 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

References

View all 15 references: Citation Explorer
Tuning the Spectroscopic Properties of Ratiometric Fluorescent Metal Indicators: Experimental and Computational Studies on Mag-Fura-2 and Analogues
Authors: Zhang G, Jacquemin D, Buccella D.
Journal: J Phys Chem B. (2017)
Fluorescence measurements of free [Mg2+] by use of mag-fura 2 in Salmonella enterica
Authors: Froschauer EM, Kolisek M, Dieterich F, Schweigel M, Schweyen RJ.
Journal: FEMS Microbiol Lett (2004): 49
Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC
Authors: Hyrc KL, Bownik JM, Goldberg MP.
Journal: Cell Calcium (2000): 75
Mag-Fura-2 (Furaptra) exhibits both low (microM) and high (nM) affinity for Ca2+
Authors: Martinez-Zaguilan R, Parnami J, Martinez GM.
Journal: Cell Physiol Biochem (1998): 158
Intracellular Mg2+ regulation in voltage-clamped Helix aspersa neurones measured with mag-fura-2 and Mg(2+)-sensitive microelectrodes
Authors: Kennedy HJ., undefined
Journal: Exp Physiol (1998): 449
Page updated on December 17, 2024

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Physical properties

Molecular weight

586.68

Solvent

Water

Spectral properties

Excitation (nm)

336

Emission (nm)

505

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200

CAS

132319-57-4
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