LysoBrite™ Green DND-26
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 362.23 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 501 |
Emission (nm) | 509 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Lysosomes
Molecular weight 362.23 | Excitation (nm) 501 | Emission (nm) 509 |
LysoBrite™ Green DND-26 is chemically the same as the LysoTracker® Green DND-26 used for labeling and tracking acidic organelles in live cells (LysoTracker® is the trademark of ThermoFisher). It has good selectivity for acidic organelles. The LysoBrite™ probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH, allowing them to freely permeate cell membranes to label live cells.
Platform
Flow cytometer
Excitation | 488 nm laser |
Emission | 530/30 nm filter |
Instrument specification(s) | FITC channel |
Fluorescence microscope
Excitation | FITC filter set |
Emission | FITC filter set |
Recommended plate | Black wall/clear bottom |
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of LysoBrite™ Green DND-26 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 276.068 µL | 1.38 mL | 2.761 mL | 13.803 mL | 27.607 mL |
5 mM | 55.214 µL | 276.068 µL | 552.135 µL | 2.761 mL | 5.521 mL |
10 mM | 27.607 µL | 138.034 µL | 276.068 µL | 1.38 mL | 2.761 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
Citations
View all 1 citations: Citation Explorer
Two-Pronged Intracellular Co-Delivery of Antigen and Adjuvant for Synergistic Cancer Immunotherapy
Authors: Meng, Junli and Zhang, Peisen and Chen, Qizhe and Wang, Zihua and Gu, Yuan and Ma, Jie and Li, Wang and Yang, Chen and Qiao, Yuanyuan and Hou, Yi and others,
Journal: Advanced Materials (2022): 2202168
Authors: Meng, Junli and Zhang, Peisen and Chen, Qizhe and Wang, Zihua and Gu, Yuan and Ma, Jie and Li, Wang and Yang, Chen and Qiao, Yuanyuan and Hou, Yi and others,
Journal: Advanced Materials (2022): 2202168
References
View all 50 references: Citation Explorer
Differential LysoTracker Uptake Defines Two Populations of Distal Epithelial Cells in Idiopathic Pulmonary Fibrosis.
Authors: Wasnick, Roxana Maria and Shalashova, Irina and Wilhelm, Jochen and Khadim, Ali and Schmidt, Nicolai and Hackstein, Holger and Hecker, Andreas and Hoetzenecker, Konrad and Seeger, Werner and Bellusci, Saverio and El Agha, Elie and Ruppert, Clemens and Guenther, Andreas
Journal: Cells (2022)
Authors: Wasnick, Roxana Maria and Shalashova, Irina and Wilhelm, Jochen and Khadim, Ali and Schmidt, Nicolai and Hackstein, Holger and Hecker, Andreas and Hoetzenecker, Konrad and Seeger, Werner and Bellusci, Saverio and El Agha, Elie and Ruppert, Clemens and Guenther, Andreas
Journal: Cells (2022)
Raman and fluorescence imaging of phospholipidosis induced by cationic amphiphilic drugs in endothelial cells.
Authors: Bik, Ewelina and Orleanska, Jagoda and Mateuszuk, Lukasz and Baranska, Malgorzata and Majzner, Katarzyna and Chlopicki, Stefan
Journal: Biochimica et biophysica acta. Molecular cell research (2022): 119186
Authors: Bik, Ewelina and Orleanska, Jagoda and Mateuszuk, Lukasz and Baranska, Malgorzata and Majzner, Katarzyna and Chlopicki, Stefan
Journal: Biochimica et biophysica acta. Molecular cell research (2022): 119186
Zeb1-mediated autophagy enhances resistance of breast cancer cells to genotoxic drugs.
Authors: Fedorova, Olga and Daks, Alexandra and Parfenyev, Sergey and Shuvalov, Oleg and Netsvetay, Sofia and Vasileva, Julia and Gudovich, Anastasia and Golotin, Vasilii and Semenov, Oleg and Petukhov, Alexey and Baiduik, Ekaterina and Berdigaliyev, Nurken and Tulchinsky, Eugene M and Barlev, Nikolai A
Journal: Biochemical and biophysical research communications (2022): 29-34
Authors: Fedorova, Olga and Daks, Alexandra and Parfenyev, Sergey and Shuvalov, Oleg and Netsvetay, Sofia and Vasileva, Julia and Gudovich, Anastasia and Golotin, Vasilii and Semenov, Oleg and Petukhov, Alexey and Baiduik, Ekaterina and Berdigaliyev, Nurken and Tulchinsky, Eugene M and Barlev, Nikolai A
Journal: Biochemical and biophysical research communications (2022): 29-34
Incidence of an Intracellular Multiplication Niche among Acinetobacter baumannii Clinical Isolates.
Authors: Rubio, Tristan and Gagné, Stéphanie and Debruyne, Charline and Dias, Chloé and Cluzel, Caroline and Mongellaz, Doriane and Rousselle, Patricia and Göttig, Stephan and Seifert, Harald and Higgins, Paul G and Salcedo, Suzana P
Journal: mSystems (2022): e0048821
Authors: Rubio, Tristan and Gagné, Stéphanie and Debruyne, Charline and Dias, Chloé and Cluzel, Caroline and Mongellaz, Doriane and Rousselle, Patricia and Göttig, Stephan and Seifert, Harald and Higgins, Paul G and Salcedo, Suzana P
Journal: mSystems (2022): e0048821
Structural Modifications to the Internal Oligoguanidinium Transporter Uncover Two Potent Analogues that Effectively Deliver the Proapoptotic Peptide in Multiple Cancer Cell Lines.
Authors: Gupta, Shalini and Qasim, Md and Gupta, Abhishek and Kundu, Jayanta and Sinha, Surajit
Journal: Bioconjugate chemistry (2022): 121-133
Authors: Gupta, Shalini and Qasim, Md and Gupta, Abhishek and Kundu, Jayanta and Sinha, Surajit
Journal: Bioconjugate chemistry (2022): 121-133
Presenilin/γ-Secretase Activity Is Located in Acidic Compartments of Live Neurons.
Authors: Maesako, Masato and Houser, Mei C Q and Turchyna, Yuliia and Wolfe, Michael S and Berezovska, Oksana
Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience (2022): 145-154
Authors: Maesako, Masato and Houser, Mei C Q and Turchyna, Yuliia and Wolfe, Michael S and Berezovska, Oksana
Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience (2022): 145-154
Synergistic effects of botanical curcumin-induced programmed cell death on the management of Spodoptera litura Fabricius with avermectin.
Authors: Cui, Gaofeng and Yuan, Haiqi and He, Wei and Deng, Yukun and Sun, Ranran and Zhong, Guohua
Journal: Ecotoxicology and environmental safety (2022): 113097
Authors: Cui, Gaofeng and Yuan, Haiqi and He, Wei and Deng, Yukun and Sun, Ranran and Zhong, Guohua
Journal: Ecotoxicology and environmental safety (2022): 113097
A monolayer hiPSC culture system for autophagy/mitophagy studies in human dopaminergic neurons.
Authors: Stathakos, Petros and Jiménez-Moreno, Natalia and Crompton, Lucy A and Nistor, Paul A and Badger, Jennifer L and Barbuti, Peter A and Kerrigan, Talitha L and Randall, Andrew D and Caldwell, Maeve A and Lane, Jon D
Journal: Autophagy (2021): 855-871
Authors: Stathakos, Petros and Jiménez-Moreno, Natalia and Crompton, Lucy A and Nistor, Paul A and Badger, Jennifer L and Barbuti, Peter A and Kerrigan, Talitha L and Randall, Andrew D and Caldwell, Maeve A and Lane, Jon D
Journal: Autophagy (2021): 855-871
In vitro cytotoxicity study of superparamagnetic iron oxide and silica nanoparticles on pneumocyte organelles.
Authors: Solorio-Rodríguez, A and Escamilla-Rivera, V and Uribe-Ramírez, M and González-Pozos, S and Hernández-Soto, J and Rafael-Vázquez, L and De Vizcaya-Ruiz, A
Journal: Toxicology in vitro : an international journal published in association with BIBRA (2021): 105071
Authors: Solorio-Rodríguez, A and Escamilla-Rivera, V and Uribe-Ramírez, M and González-Pozos, S and Hernández-Soto, J and Rafael-Vázquez, L and De Vizcaya-Ruiz, A
Journal: Toxicology in vitro : an international journal published in association with BIBRA (2021): 105071
Late endosomal/lysosomal accumulation of a neurotransmitter receptor in a cellular model of Smith-Lemli-Opitz syndrome.
Authors: Sharma, Ashwani and Kumar, G Aditya and Chattopadhyay, Amitabha
Journal: Traffic (Copenhagen, Denmark) (2021): 332-344
Authors: Sharma, Ashwani and Kumar, G Aditya and Chattopadhyay, Amitabha
Journal: Traffic (Copenhagen, Denmark) (2021): 332-344
Application notes
FAQ
Can I use any of your Cell Meter™ Autophagy Assay Kits with a flow cytometer?
How do cells protect themselves against cellular stress?
How do I label the cell membrane?
How does DiI reach internal membranous organelles in the fixed cells?
How does the autophagy marker in your Cell Meter™ Autophagy Assay Kit (Cat#23000) work?
How do cells protect themselves against cellular stress?
How do I label the cell membrane?
How does DiI reach internal membranous organelles in the fixed cells?
How does the autophagy marker in your Cell Meter™ Autophagy Assay Kit (Cat#23000) work?