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Lucigenin [Bis-N-methylacridinium nitrate]
A chloride indicator that has the fluorescence dependent of chloride concentration. Its fluorescence is quenched by chloride through collision quenching. In addition, it is also used for detecting superoxide.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Lucigenin [Bis-N-methylacridinium nitrate] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 195.886 µL | 979.432 µL | 1.959 mL | 9.794 mL | 19.589 mL |
| 5 mM | 39.177 µL | 195.886 µL | 391.773 µL | 1.959 mL | 3.918 mL |
| 10 mM | 19.589 µL | 97.943 µL | 195.886 µL | 979.432 µL | 1.959 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
References
View all 33 references: Citation Explorer
Flow injection chemiluminescence determination of isoniazid using the lucigenin-periodate system
Authors: Du J, Lu J.
Journal: Luminescence (2006): 26
Authors: Du J, Lu J.
Journal: Luminescence (2006): 26
Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa
Authors: Baker MA, Krutskikh A, Curry BJ, Hetherington L, Aitken RJ.
Journal: Biol Reprod (2005): 334
Authors: Baker MA, Krutskikh A, Curry BJ, Hetherington L, Aitken RJ.
Journal: Biol Reprod (2005): 334
Chemiluminescence of lucigenin is dependent on experimental conditions
Authors: Hyrsl P, Lojek A, Ciz M, Kubala L.
Journal: Luminescence (2004): 61
Authors: Hyrsl P, Lojek A, Ciz M, Kubala L.
Journal: Luminescence (2004): 61
Luminol-, isoluminol- and lucigenin-enhanced chemiluminescence of rat blood phagocytes stimulated with different activators
Authors: Pavelkova M, Kubala L.
Journal: Luminescence (2004): 37
Authors: Pavelkova M, Kubala L.
Journal: Luminescence (2004): 37
Lucigenin and coelenterazine as superoxide probes in mitochondrial and bacterial membranes
Authors: Kervinen M, Patsi J, Finel M, Hassinen IE.
Journal: Anal Biochem (2004): 45
Authors: Kervinen M, Patsi J, Finel M, Hassinen IE.
Journal: Anal Biochem (2004): 45
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