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Luciferase *Recombinant firefly*

This enzyme is a luciferase expressed from a cloned gene from the North American firefly (Photinus pyralis) that provides the reliability and dependability needed for performing research or producing kits using bioluminescence reagents to detect ATP or luciferin substrates. This recombinant enzyme can potentially eliminate the possibility of seasonal and regional variability that may be found in luciferase purified from natural sources.

References

View all 40 references: Citation Explorer
Measuring HIV Neutralization in a Luciferase Reporter Gene Assay
Authors: Montefiori DC., undefined
Journal: Methods Mol Biol (2009): 395
An optimized luciferase bioluminescent assay for coenzyme A
Authors: Marques SM, da Silva JC.
Journal: Anal Bioanal Chem (2008): 2161
Quantitative assessment of the knockdown efficiency of morpholino antisense oligonucleotides in zebrafish embryos using a luciferase assay
Authors: Kamachi Y, Okuda Y, Kondoh H.
Journal: Genesis (2008): 1
A cell-based luciferase assay amenable to high-throughput screening of inhibitors of arenavirus budding
Authors: Capul AA, de la Torre JC.
Journal: Virology (2008): 107
Application of the dual-luciferase reporter assay to the analysis of promoter activity in Zebrafish embryos
Authors: Alcaraz-Perez F, Mulero V, Cayuela ML.
Journal: BMC Biotechnol (2008): 81
Page updated on October 28, 2024

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Catalog Number12500
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Physical properties

Molecular weight

~60 kDa

Solvent

Water

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC41105331

CAS

61970-00-1
Luciferase activity was measured with Amplite® Luciferase Reporter Gene Assay Kit in a white 96-well plate with a NOVOstar plate reader (BMG Labtech). The luciferase activity is still detectable at 0.1 pg/well with 20 minutes incubation.
Luciferase activity was measured with Amplite® Luciferase Reporter Gene Assay Kit in a white 96-well plate with a NOVOstar plate reader (BMG Labtech). The luciferase activity is still detectable at 0.1 pg/well with 20 minutes incubation.
Luciferase activity was measured with Amplite® Luciferase Reporter Gene Assay Kit in a white 96-well plate with a NOVOstar plate reader (BMG Labtech). The luciferase activity is still detectable at 0.1 pg/well with 20 minutes incubation.
Comparison of the heat stability of Luciferase *Recombinant firefly* and &nbsp;Luciferase(from Vendor A) after&nbsp;incubation at 60 &deg;C.