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Leu-AMC [L-Leucine-7-amido-4-methylcoumarin] *CAS 62480-44-8*

Leu-AMC is a fluorogenic substrate for leucine aminopeptidase. Upon the hydrolysis by proteases, Leu-AMC releases the strongly fluorescent AMC fluorophore.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Leu-AMC [L-Leucine-7-amido-4-methylcoumarin] *CAS 62480-44-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM307.882 µL1.539 mL3.079 mL15.394 mL30.788 mL
5 mM61.576 µL307.882 µL615.764 µL3.079 mL6.158 mL
10 mM30.788 µL153.941 µL307.882 µL1.539 mL3.079 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)
Pro-AMC341441
Ala-AMC341441

References

View all 39 references: Citation Explorer
Molecular and biochemical characterization of methionine aminopeptidase of Babesia bovis as a potent drug target
Authors: Munkhjargal T, Ishizaki T, Guswanto A, Takemae H, Yokoyama N, Igarashi I.
Journal: Vet Parasitol (2016): 14
Secretory Expression, Purification, Characterization, and Application of an Aspergillus oryzae Prolyl Aminopeptidase in Bacillus subtilis
Authors: Wang KD, Wang KH, Zhou ND, Tian YP.
Journal: Appl Biochem Biotechnol. (2016)
Inhibition of leucine aminopeptidase 3 suppresses invasion of ovarian cancer cells through down-regulation of fascin and MMP-2/9
Authors: Wang X, Shi L, Deng Y, Qu M, Mao S, Xu L, Xu W, Fang C.
Journal: Eur J Pharmacol (2015): 116
Essential Role for an M17 Leucine Aminopeptidase in Encystation of Acanthamoeba castellanii
Authors: Lee YR, Na BK, Moon EK, Song SM, Joo SY, Kong HH, Goo YK, Chung DI, Hong Y.
Journal: PLoS One (2015): e0129884
Molecular Characterization of Babesia bovis M17 Leucine Aminopeptidase and Inhibition of Babesia Growth by Bestatin
Authors: Aboge GO, Cao S, Terkawi MA, Masatani T, Goo Y, AbouLaila M, Nishikawa Y, Igarashi I, Suzuki H, Xuan X.
Journal: J Parasitol (2015): 536
Page updated on November 12, 2024

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Physical properties

Molecular weight

324.80

Solvent

DMSO

Spectral properties

Excitation (nm)

341

Emission (nm)

441

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200

CAS

62480-44-8
L-Leucine-7-amido-4-methylcoumarin
L-Leucine-7-amido-4-methylcoumarin
L-Leucine-7-amido-4-methylcoumarin