iFluor® 594 Tetrazine
Ordering information
Price | |
Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1158.21 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 587 |
Correction Factor (260 nm) | 0.05 |
Correction Factor (280 nm) | 0.04 |
Extinction coefficient (cm -1 M -1) | 2000001 |
Excitation (nm) | 587 |
Emission (nm) | 603 |
Quantum yield | 0.531 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: iFluor® Dyes and Kits
Molecular weight 1158.21 | Absorbance (nm) 587 | Correction Factor (260 nm) 0.05 | Correction Factor (280 nm) 0.04 | Extinction coefficient (cm -1 M -1) 2000001 | Excitation (nm) 587 | Emission (nm) 603 | Quantum yield 0.531 |
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 594 tetrazine can be readily used to label TCO-modified biological molecules for fluorescence imaging and other fluorescence-based biological applications. The conjugates prepared with iFluor® 594 dye have spectral properties almost identical to the popular Cy3.5 and Alexa Fluor® 594. In most cases, antibody conjugates prepared with iFluor® 594 tend to have lower background than the spectrally similar dye conjugates, such as Cy3.5 and Alexa Fluor® 594 (Alexa Fluor® is the trademark of Invitrogen).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 594 Tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 86.34 µL | 431.701 µL | 863.401 µL | 4.317 mL | 8.634 mL |
5 mM | 17.268 µL | 86.34 µL | 172.68 µL | 863.401 µL | 1.727 mL |
10 mM | 8.634 µL | 43.17 µL | 86.34 µL | 431.701 µL | 863.401 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fifluor_594.png&w=2048&q=50)
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fifluor_594.png&w=2048&q=50)
Spectral properties
Absorbance (nm) | 587 |
Correction Factor (260 nm) | 0.05 |
Correction Factor (280 nm) | 0.04 |
Extinction coefficient (cm -1 M -1) | 2000001 |
Excitation (nm) | 587 |
Emission (nm) | 603 |
Quantum yield | 0.531 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 594 maleimide | 587 | 603 | 2000001 | 0.531 | 0.05 | 0.04 |
iFluor® 594 Styramide *Superior Replacement for Alexa Fluor 594 tyramide* | 587 | 603 | 2000001 | 0.531 | 0.05 | 0.04 |
iFluor® 594 Tyramide | 587 | 603 | 2000001 | 0.531 | 0.05 | 0.04 |
iFluor® 594 TCO | 587 | 603 | 2000001 | 0.531 | 0.05 | 0.04 |
iFluor® 488 Tetrazine | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555 Tetrazine | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 647 Tetrazine | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
Images
![The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 594 tetrazine can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fifluor-594-tetrazine%2Ffigure-for-ifluor-594-tetrazine_GeuO2.jpg&w=3840&q=75)
Figure 1. The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 594 tetrazine can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.
References
View all 50 references: Citation Explorer
Facile Click-Mediated Cell Imaging Strategy of Liposomal Azido Mannosamine Lipids via Metabolic or Nonmetabolic Glycoengineering.
Authors: Shen, Li and Cai, Kaimin and Yu, Jin and Cheng, Jianjun
Journal: ACS omega (2020): 14111-14115
Authors: Shen, Li and Cai, Kaimin and Yu, Jin and Cheng, Jianjun
Journal: ACS omega (2020): 14111-14115
Bioorthogonal Labeling Reveals Different Expression of Glycans in Mouse Hippocampal Neuron Cultures during Their Development.
Authors: Soares da Costa, Diana and Sousa, João C and Dá Mesquita, Sandro and Petkova-Yankova, Nevena I and Marques, Fernanda and Reis, Rui L and Sousa, Nuno and Pashkuleva, Iva
Journal: Molecules (Basel, Switzerland) (2020)
Authors: Soares da Costa, Diana and Sousa, João C and Dá Mesquita, Sandro and Petkova-Yankova, Nevena I and Marques, Fernanda and Reis, Rui L and Sousa, Nuno and Pashkuleva, Iva
Journal: Molecules (Basel, Switzerland) (2020)
Surface-modified nanoerythrosomes for potential optical imaging diagnostics.
Authors: Fornasier, Marco and Porcheddu, Andrea and Casu, Anna and Raghavan, Srinivasa R and Jönsson, Peter and Schillén, Karin and Murgia, Sergio
Journal: Journal of colloid and interface science (2020): 246-253
Authors: Fornasier, Marco and Porcheddu, Andrea and Casu, Anna and Raghavan, Srinivasa R and Jönsson, Peter and Schillén, Karin and Murgia, Sergio
Journal: Journal of colloid and interface science (2020): 246-253
Novel Liposomal Azido Mannosamine Lipids on Metabolic Cell Labeling and Imaging via Cu-Free Click Chemistry.
Authors: Shen, Li and Cai, Kaimin and Yu, Jin and Cheng, Jianjun
Journal: Bioconjugate chemistry (2019): 2317-2322
Authors: Shen, Li and Cai, Kaimin and Yu, Jin and Cheng, Jianjun
Journal: Bioconjugate chemistry (2019): 2317-2322
One-Pot Synthesis of Triazolobenzodiazepines Through Decarboxylative [3 + 2] Cycloaddition of Nonstabilized Azomethine Ylides and Cu-Free Click Reactions.
Authors: Ma, Xiaoming and Zhang, Xiaofeng and Qiu, Weiqi and Zhang, Wensheng and Wan, Bruce and Evans, Jason and Zhang, Wei
Journal: Molecules (Basel, Switzerland) (2019)
Authors: Ma, Xiaoming and Zhang, Xiaofeng and Qiu, Weiqi and Zhang, Wensheng and Wan, Bruce and Evans, Jason and Zhang, Wei
Journal: Molecules (Basel, Switzerland) (2019)
Correction to "Photochemical Formation of Dibenzosilacyclohept-4-yne for Cu-Free Click Chemistry with Azides and 1,2,4,5-Tetrazines".
Authors: Martínek, Marek and Filipová, Lenka and Galeta, Juraj and Ludvíková, Lucie and Klán, Petr
Journal: Organic letters (2019): 8506
Authors: Martínek, Marek and Filipová, Lenka and Galeta, Juraj and Ludvíková, Lucie and Klán, Petr
Journal: Organic letters (2019): 8506
Targeting Mutated Plus Germline Epitopes Confers Pre-clinical Efficacy of an Instantly Formulated Cancer Nano-Vaccine.
Authors: Mohsen, Mona O and Vogel, Monique and Riether, Carsten and Muller, Julius and Salatino, Silvia and Ternette, Nicola and Gomes, Ariane C and Cabral-Miranda, Gustavo and El-Turabi, Aadil and Ruedl, Christiane and Kundig, Thomas M and Dermime, Said and Knuth, Alexander and Speiser, Daniel E and Bachmann, Martin F
Journal: Frontiers in immunology (2019): 1015
Authors: Mohsen, Mona O and Vogel, Monique and Riether, Carsten and Muller, Julius and Salatino, Silvia and Ternette, Nicola and Gomes, Ariane C and Cabral-Miranda, Gustavo and El-Turabi, Aadil and Ruedl, Christiane and Kundig, Thomas M and Dermime, Said and Knuth, Alexander and Speiser, Daniel E and Bachmann, Martin F
Journal: Frontiers in immunology (2019): 1015
Vaccination with nanoparticles combined with micro-adjuvants protects against cancer.
Authors: Mohsen, Mona O and Heath, Matthew D and Cabral-Miranda, Gustavo and Lipp, Cyrill and Zeltins, Andris and Sande, Marcos and Stein, Jens V and Riether, Carsten and Roesti, Elisa and Zha, Lisha and Engeroff, Paul and El-Turabi, Aadil and Kundig, Thomas M and Vogel, Monique and Skinner, Murray A and Speiser, Daniel E and Knuth, Alexander and Kramer, Matthias F and Bachmann, Martin F
Journal: Journal for immunotherapy of cancer (2019): 114
Authors: Mohsen, Mona O and Heath, Matthew D and Cabral-Miranda, Gustavo and Lipp, Cyrill and Zeltins, Andris and Sande, Marcos and Stein, Jens V and Riether, Carsten and Roesti, Elisa and Zha, Lisha and Engeroff, Paul and El-Turabi, Aadil and Kundig, Thomas M and Vogel, Monique and Skinner, Murray A and Speiser, Daniel E and Knuth, Alexander and Kramer, Matthias F and Bachmann, Martin F
Journal: Journal for immunotherapy of cancer (2019): 114
DNA-Functionalized Dye-Loaded Polymeric Nanoparticles: Ultrabright FRET Platform for Amplified Detection of Nucleic Acids.
Authors: Melnychuk, Nina and Klymchenko, Andrey S
Journal: Journal of the American Chemical Society (2018): 10856-10865
Authors: Melnychuk, Nina and Klymchenko, Andrey S
Journal: Journal of the American Chemical Society (2018): 10856-10865
64Cu-Labeled Phosphonate Cross-Bridged Chelator Conjugates of c(RGDyK) for PET/CT Imaging of Osteolytic Bone Metastases.
Authors: Ocak, Meltem and Beaino, Wissam and White, Alexander and Zeng, Dexing and Cai, Zhengxin and Anderson, Carolyn J
Journal: Cancer biotherapy & radiopharmaceuticals (2018): 74-83
Authors: Ocak, Meltem and Beaino, Wissam and White, Alexander and Zeng, Dexing and Cai, Zhengxin and Anderson, Carolyn J
Journal: Cancer biotherapy & radiopharmaceuticals (2018): 74-83