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iFluor® 555 Tetrazine
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 555 tetrazine can be readily used to label TCO-modified biological molecules for fluorescence imaging and other fluorescence-based biological applications. The peptide and oligo conjugates prepared with iFluor® 555 dye are far superior to the conjugates of other spectrally similar dyes, such as the popular Cy3 and Alexa Fluor® 555. iFluor® 555 conjugates are significantly brighter and much more photostable than the corresponding conjugates of Cy3 and Alexa Fluor® 555 (Alexa Fluor® is the trademark of Invitrogen).
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| iFluor® 555 maleimide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 amine | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 hydrazide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 alkyne | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 azide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 Styramide *Superior Replacement for Alexa Fluor 555 tyramide and Opal 570* | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 Tyramide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 TCO | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 488 Tetrazine | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
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References
View all 33 references: Citation Explorer
Nanobody click chemistry for convenient site-specific fluorescent labelling, single step immunocytochemistry and delivery into living cells by photoporation and live cell imaging.
Authors: Hebbrecht, Tim and Liu, Jing and Zwaenepoel, Olivier and Boddin, Gaëlle and Van Leene, Chloé and Decoene, Klaas and Madder, Annemieke and Braeckmans, Kevin and Gettemans, Jan
Journal: New biotechnology (2020): 33-43
Authors: Hebbrecht, Tim and Liu, Jing and Zwaenepoel, Olivier and Boddin, Gaëlle and Van Leene, Chloé and Decoene, Klaas and Madder, Annemieke and Braeckmans, Kevin and Gettemans, Jan
Journal: New biotechnology (2020): 33-43
A novel tracer for in vivo optical imaging of fatty acid metabolism in the heart and brown adipose tissue.
Authors: Panagia, Marcello and Yang, Jing and Gale, Eric and Wang, Huan and Luptak, Ivan and Chen, Howard H and Patel, Dakshesh and Croteau, Dominique and Pimentel, David Richard and Bachschmid, Markus Michael and Colucci, Wilson S and Ran, Chongzhao and Sosnovik, David E
Journal: Scientific reports (2020): 11209
Authors: Panagia, Marcello and Yang, Jing and Gale, Eric and Wang, Huan and Luptak, Ivan and Chen, Howard H and Patel, Dakshesh and Croteau, Dominique and Pimentel, David Richard and Bachschmid, Markus Michael and Colucci, Wilson S and Ran, Chongzhao and Sosnovik, David E
Journal: Scientific reports (2020): 11209
Tylophorine reduces protein biosynthesis and rapidly decreases cyclin D1, inhibiting vascular smooth muscle cell proliferation in vitro and in organ culture.
Authors: Joa, Helge and Blažević, Tina and Grojer, Christoph and Zeller, Iris and Heiss, Elke H and Atanasov, Atanas G and Feldler, Ines and Gruzdaitis, Päivi and Czaloun, Christa and Proksch, Peter and Messner, Barbara and Bernhard, David and Dirsch, Verena M
Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (2019): 152938
Authors: Joa, Helge and Blažević, Tina and Grojer, Christoph and Zeller, Iris and Heiss, Elke H and Atanasov, Atanas G and Feldler, Ines and Gruzdaitis, Päivi and Czaloun, Christa and Proksch, Peter and Messner, Barbara and Bernhard, David and Dirsch, Verena M
Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (2019): 152938
PET imaging of EGFR expression using an 18F-labeled RNA aptamer.
Authors: Cheng, Siyuan and Jacobson, Orit and Zhu, Guizhi and Chen, Zhen and Liang, Steve H and Tian, Rui and Yang, Zhen and Niu, Gang and Zhu, Xiaohua and Chen, Xiaoyuan
Journal: European journal of nuclear medicine and molecular imaging (2019): 948-956
Authors: Cheng, Siyuan and Jacobson, Orit and Zhu, Guizhi and Chen, Zhen and Liang, Steve H and Tian, Rui and Yang, Zhen and Niu, Gang and Zhu, Xiaohua and Chen, Xiaoyuan
Journal: European journal of nuclear medicine and molecular imaging (2019): 948-956
Long-term live cells observation of internalized fluorescent Fe@C nanoparticles in constant magnetic field.
Authors: Garanina, Anastasiia and Kireev, Igor and Zhironkina, Oxana and Strelkova, Olga and Shakhov, Anton and Alieva, Irina and Davydov, Valery and Murugesan, Sankaran and Khabashesku, Valery and Majouga, Alexander and Agafonov, Viatcheslav and Uzbekov, Rustem
Journal: Journal of nanobiotechnology (2019): 27
Authors: Garanina, Anastasiia and Kireev, Igor and Zhironkina, Oxana and Strelkova, Olga and Shakhov, Anton and Alieva, Irina and Davydov, Valery and Murugesan, Sankaran and Khabashesku, Valery and Majouga, Alexander and Agafonov, Viatcheslav and Uzbekov, Rustem
Journal: Journal of nanobiotechnology (2019): 27
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