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iFluor® 555 TCO
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1236.49 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.23 |
| Correction Factor (280 nm) | 0.14 |
| Extinction coefficient (cm -1 M -1) | 1000001 |
| Excitation (nm) | 557 |
| Emission (nm) | 570 |
| Quantum yield | 0.641 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
See also: iFluor® Dyes and Kits
Molecular weight 1236.49 | Correction Factor (260 nm) 0.23 | Correction Factor (280 nm) 0.14 | Extinction coefficient (cm -1 M -1) 1000001 | Excitation (nm) 557 | Emission (nm) 570 | Quantum yield 0.641 |
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 555-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biological applications. The peptide and oligo conjugates prepared with iFluor® 555 dye are far superior to the conjugates of other spectrally similar dyes, such as the popular Cy3 and Alexa Fluor® 555. iFluor® 555 conjugates are significantly brighter and much more photostable than the corresponding conjugates of Cy3 and Alexa Fluor® 555 (Alexa Fluor® is the trademark of Invitrogen).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 555 TCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 80.874 µL | 404.37 µL | 808.741 µL | 4.044 mL | 8.087 mL |
| 5 mM | 16.175 µL | 80.874 µL | 161.748 µL | 808.741 µL | 1.617 mL |
| 10 mM | 8.087 µL | 40.437 µL | 80.874 µL | 404.37 µL | 808.741 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.23 |
| Correction Factor (280 nm) | 0.14 |
| Extinction coefficient (cm -1 M -1) | 1000001 |
| Excitation (nm) | 557 |
| Emission (nm) | 570 |
| Quantum yield | 0.641 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| iFluor® 555 maleimide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 amine | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 hydrazide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 alkyne | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 azide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 Styramide *Superior Replacement for Alexa Fluor 555 tyramide and Opal 570* | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 555 Tyramide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 488 TCO | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
| iFluor® 594 TCO | 587 | 603 | 2000001 | 0.531 | 0.05 | 0.04 |
Show More (2) | ||||||
Images
Figure 1. The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 555 TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.
References
View all 50 references: Citation Explorer
Novel Fluorescent Labeled Octasilsesquioxanes Nanohybrids as Potential Materials for Latent Fingerprinting Detection.
Authors: O Dare, Enock and Vendrell-Criado, Victoria and Jiménez, M Consuelo and Perez-Ruiz, Raúl and Díaz Díaz, David
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)
Authors: O Dare, Enock and Vendrell-Criado, Victoria and Jiménez, M Consuelo and Perez-Ruiz, Raúl and Díaz Díaz, David
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)
Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes.
Authors: Stone, M Rhia L and Phetsang, Wanida and Cooper, Matthew A and Blaskovich, Mark A T
Journal: Journal of visualized experiments : JoVE (2020)
Authors: Stone, M Rhia L and Phetsang, Wanida and Cooper, Matthew A and Blaskovich, Mark A T
Journal: Journal of visualized experiments : JoVE (2020)
Target verification of artesunate-related antiviral drugs: Assessing the role of mitochondrial and regulatory proteins by click chemistry and fluorescence labeling.
Authors: Hahn, Friedrich and Niesar, Aischa and Wangen, Christina and Wild, Markus and Grau, Benedikt and Herrmann, Lars and Capci, Aysun and Adrait, Annie and Couté, Yohann and Tsogoeva, Svetlana B and Marschall, Manfred
Journal: Antiviral research (2020): 104861
Authors: Hahn, Friedrich and Niesar, Aischa and Wangen, Christina and Wild, Markus and Grau, Benedikt and Herrmann, Lars and Capci, Aysun and Adrait, Annie and Couté, Yohann and Tsogoeva, Svetlana B and Marschall, Manfred
Journal: Antiviral research (2020): 104861
A novel polythioether-based rhodamine B fluorescent probe via successive click reaction and its application in iron ion detection and cell imaging.
Authors: Zhang, Xiaomei and Gou, Zhiming and Zuo, Yujing and Lin, Weiying
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2020): 117679
Authors: Zhang, Xiaomei and Gou, Zhiming and Zuo, Yujing and Lin, Weiying
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2020): 117679
A glutathione-responsive photosensitizer with fluorescence resonance energy transfer characteristics for imaging-guided targeting photodynamic therapy.
Authors: Cao, Jing-Jing and Zhang, Ming-Shan and Li, Xiao-Qiang and Yang, De-Chao and Xu, Gan and Liu, Jian-Yong
Journal: European journal of medicinal chemistry (2020): 112203
Authors: Cao, Jing-Jing and Zhang, Ming-Shan and Li, Xiao-Qiang and Yang, De-Chao and Xu, Gan and Liu, Jian-Yong
Journal: European journal of medicinal chemistry (2020): 112203
In cellulo generation of fluorescent probes for live-cell imaging of cylooxygenase-2.
Authors: Kaur, Jatinder and Bhardwaj, Atul and Wuest, Frank
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)
Authors: Kaur, Jatinder and Bhardwaj, Atul and Wuest, Frank
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)
A Systematic Study of Coumarin-Tetrazine Light-Up Probes for Bioorthogonal Fluorescence Imaging.
Authors: Galeta, Juraj and Dzijak, Rastislav and Obořil, Jan and Dračínský, Martin and Vrabel, Milan
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)
Authors: Galeta, Juraj and Dzijak, Rastislav and Obořil, Jan and Dračínský, Martin and Vrabel, Milan
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)
Fluorescent Properties of Carboxyfluorescein Bifluorophores.
Authors: Povedailo, Vladimir A and Lysenko, Ivan L and Tikhomirov, Sergei A and Yakovlev, Dmitrii L and Tsybulsky, Dmitry A and Kruhlik, Aliaksandr S and Fan, Fan and Martynenko-Makaev, Yury V and Sharko, Olga L and Duong, Pham V and Minh, Pham H and Shmanai, Vadim V
Journal: Journal of fluorescence (2020): 629-635
Authors: Povedailo, Vladimir A and Lysenko, Ivan L and Tikhomirov, Sergei A and Yakovlev, Dmitrii L and Tsybulsky, Dmitry A and Kruhlik, Aliaksandr S and Fan, Fan and Martynenko-Makaev, Yury V and Sharko, Olga L and Duong, Pham V and Minh, Pham H and Shmanai, Vadim V
Journal: Journal of fluorescence (2020): 629-635
In Situ One-Step Fluorescence Labeling Strategy of Exosomes via Bioorthogonal Click Chemistry for Real-Time Exosome Tracking In Vitro and In Vivo.
Authors: Song, Sukyung and Shim, Man Kyu and Lim, Seungho and Moon, Yujeong and Yang, Suah and Kim, Jinseong and Hong, Yeonsun and Yoon, Hong Yeol and Kim, In-San and Hwang, Kwang Yeon and Kim, Kwangmeyung
Journal: Bioconjugate chemistry (2020): 1562-1574
Authors: Song, Sukyung and Shim, Man Kyu and Lim, Seungho and Moon, Yujeong and Yang, Suah and Kim, Jinseong and Hong, Yeonsun and Yoon, Hong Yeol and Kim, In-San and Hwang, Kwang Yeon and Kim, Kwangmeyung
Journal: Bioconjugate chemistry (2020): 1562-1574
Aptamer/magnetic nanoparticles decorated with fluorescent gold nanoclusters for selective detection and collection of human promyelocytic leukemia (HL-60) cells from a mixture.
Authors: Haghighi, Farid Hajareh and Binaymotlagh, Roya and Mirahmadi-Zare, Seyede Zohreh and Hadadzadeh, Hassan
Journal: Nanotechnology (2020): 025605
Authors: Haghighi, Farid Hajareh and Binaymotlagh, Roya and Mirahmadi-Zare, Seyede Zohreh and Hadadzadeh, Hassan
Journal: Nanotechnology (2020): 025605