iFluor® 488 Anti-human CD45 Antibody *AHC0057*

iFluor® 488 Anti-human CD45 Antibody

AHC0057

AHC0057 is an anti-human monoclonal antibody that recognizes the CD45 antigen. CD45 (sometimes called T200 or Leukocyte Common Antigen (LCA)) is a 180 - 240 kD transmembrane glycoprotein that is located on the surface of cells such as granulocytes, stem cells, B cells and NK cells. CD45 has been thought to be involved with important biological processes like dephosphorylation, specifically protein dephosphorylation. Moreover, in many organisms, it plays a role in the downregulation of microglial cell activation, is involved in the negative regulation of protein autophosphorylation and is a promoter of T cell mediated cytotoxicity. CD45 acts in important cellular pathways, namely, the positive regulation of extrinsic apoptotic signaling pathway, negative regulation of cytokine-mediated signaling pathway and B cell receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with critical macromolecules/ligands such as src kinases and p59fyn. CD45 is a very popular antibody target, with over 54000 publications in the last decade. CD45 is essential for cell biology research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 488 (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 530/30 nm bandpass filter (for example, as in the BD FACSAria™ III).

Protocol

COA

Catalog	Size	Price	Quantity
10457050	100 tests	Price
10457051	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd45 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	Leukocyte Common Antigen (LCA); T200; PTPRC
Clone	AHC0057
Host	mouse
Isotype	igg2a
Reactivity	human
Application	Flow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Documents

Protocol
Safety Data Sheet
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on August 5, 2024