iFluor® 488 Anti-human CD3 Antibody *HIT3a*

HIT3a is an anti-human monoclonal antibody that recognizes the CD3e antigen. CD3e (sometimes referred to as T cell antigen receptor complex or T3E) is a 20 kD member of the Ig superfamily that is found on the surface of cells like T cells. CD3 acts in essential cellular pathways, namely, the G protein-coupled receptor signaling pathway, apoptotic signaling pathway and T cell receptor signaling pathway. In addition, in certain organisms, it positively regulates T cell anergy, acts to positively regulate cell-matrix adhesion and is involved in the positive regulation of calcium-mediated signaling. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands such as TCR. CD3 is a very popular antibody target, with over 80000 publications in the last decade. CD3e is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 488 (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 530/30 nm bandpass filter (for example, as in the BD FACSCanto™ II).

Page updated on November 21, 2024