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IDA maleimide
Immobilized metal affinity chromatography (IMAC) is a popular method for protein purification, particularly for recombinant proteins fused to a polyhistidine-tag. Transition metal ions immobilized to a matrix through a chelating ligand interact with the polyhistidine-tag, effectively sequestering the fused protein from a sample. Nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA) are two such ligands commonly used in commercially available resins. AAT Bioquest offer a variety of NTA building blocks for developing either NTA-based purification and detection. IDA is complimentary to NTA. The tridentate IDA ligand requires a lower imidazole concentration to elute protein than the tetradentate NTA. IDA is a smaller molecule which can be coupled to the matrix at a higher density resulting in a higher metal loading capacity. IDA maleimide is an excellent building block that can be used to develop a variety of IDA probes for detecting polyHis-containing proteins, and tools for purifying polyHis tagged proteins.
References
View all 28 references: Citation Explorer
Heterologous overexpression of active hexokinases from microsporidia Nosema bombycis and Nosema ceranae confirms their ability to phosphorylate host glucose.
Authors: Dolgikh, Viacheslav V and Tsarev, Alexander A and Timofeev, Sergey A and Zhuravlyov, Vladimir S
Journal: Parasitology research (2019): 1511-1518
Authors: Dolgikh, Viacheslav V and Tsarev, Alexander A and Timofeev, Sergey A and Zhuravlyov, Vladimir S
Journal: Parasitology research (2019): 1511-1518
Systematic analysis of the expression, solubility and purification of a passenger protein in fusion with different tags.
Authors: Bernier, Sarah C and Cantin, Line and Salesse, Christian
Journal: Protein expression and purification (2018): 92-106
Authors: Bernier, Sarah C and Cantin, Line and Salesse, Christian
Journal: Protein expression and purification (2018): 92-106
Production of a truncated recombinant HA1 for influenza A H9 subtype screening.
Authors: Tombari, Wafa and Ghram, Abdeljelil
Journal: Biologicals : journal of the International Association of Biological Standardization (2016): 546-555
Authors: Tombari, Wafa and Ghram, Abdeljelil
Journal: Biologicals : journal of the International Association of Biological Standardization (2016): 546-555
Characterization of the Eimeria maxima sporozoite surface protein IMP1.
Authors: Jenkins, M C and Fetterer, R and Miska, K and Tuo, W and Kwok, O and Dubey, J P
Journal: Veterinary parasitology (2015): 146-52
Authors: Jenkins, M C and Fetterer, R and Miska, K and Tuo, W and Kwok, O and Dubey, J P
Journal: Veterinary parasitology (2015): 146-52
Effects of cholesterol incorporation on the physicochemical, colloidal, and biological characteristics of pH-sensitive AB₂ miktoarm polymer-based polymersomes.
Authors: Yin, Haiqing and Kang, Han Chang and Huh, Kang Moo and Bae, You Han
Journal: Colloids and surfaces. B, Biointerfaces (2014): 128-37
Authors: Yin, Haiqing and Kang, Han Chang and Huh, Kang Moo and Bae, You Han
Journal: Colloids and surfaces. B, Biointerfaces (2014): 128-37
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