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HRP-streptavidin conjugate
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This HRP-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with HRP covalently attached (as the enzyme label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with a HRP substrate (such as our sensitive fluorogenic ADHP peroxidase substrate). It is a very valuable tool for biotin-streptavidin-based biological assays and tests. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.
Citations
View all 3 citations: Citation Explorer
Therapeutic efficacy of intracerebral hematopoietic stem cell gene therapy in an Alzheimer’s disease mouse model
Authors: Milazzo, Rita and Montepeloso, Annita and Kumar, Rajesh and Ferro, Francesca and Cavalca, Eleonora and Rigoni, Pietro and Cabras, Paolo and Ciervo, Yuri and Das, Sabyasachi and Capotondo, Alessia and others,
Journal: Nature Communications (2024): 8024
Authors: Milazzo, Rita and Montepeloso, Annita and Kumar, Rajesh and Ferro, Francesca and Cavalca, Eleonora and Rigoni, Pietro and Cabras, Paolo and Ciervo, Yuri and Das, Sabyasachi and Capotondo, Alessia and others,
Journal: Nature Communications (2024): 8024
Enzyme-linked oligonucleotide hybridization assay for direct oligo measurement in blood
Authors: Lorenson, Mary Y and Chen, Kuan-Hui Ethan and Walker, Ameae M
Journal: Biology Methods and Protocols (2019): bpy014
Authors: Lorenson, Mary Y and Chen, Kuan-Hui Ethan and Walker, Ameae M
Journal: Biology Methods and Protocols (2019): bpy014
Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996
References
View all 47 references: Citation Explorer
A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647
Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50
Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label
Authors: Sun M, Du L, Gao S, Bao Y, Wang S.
Journal: Steroids (2010): 400
Authors: Sun M, Du L, Gao S, Bao Y, Wang S.
Journal: Steroids (2010): 400
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