Horseradish Peroxidase *Optimized for conjugation*
Horseradish peroxidase (HRP) is a glycoprotein with a molecular weight of ~44 KD. It is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine. HRP readily combines with hydrogen peroxide (H2O2), and the resultant [HRP–H2O2] complex can oxidize a wide variety of chromogenic hydrogen donors. AAT Bioquest offers the largest collection of HRP substrates and conjugates. HRP is widely used as a label for immunoglobulins in many different immunochemistry applications, including ELISA, immunoblotting and immunohistochemistry. HRP can be conjugated to antibodies by several different methods, including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed cross-inkers. Our Buccutite™ HRP conjugation technique is by far the most effective method. HRP is the most desired label for antibodies since it is the smallest and most stable of the three most popular enzyme labels (HRP, alkaline phosphatase, and B-galactosidase), and its glycosylation leads to lower non-specific binding. This purified horseradish peroxidase (HRP) is supplied lyophilized as a salt-free powder for reconstitution and use in HRP detections or conjugations.
References
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Nanocapsulation of horseradish peroxidase (HRP) enhances enzymatic performance in removing phenolic compounds.
Authors: Liu, Shan and Huang, Biyan and Zheng, Guiqin and Zhang, Peng and Li, Juying and Yang, Bo and Chen, Yantao and Liang, Li
Journal: International journal of biological macromolecules (2020): 814-822
Authors: Liu, Shan and Huang, Biyan and Zheng, Guiqin and Zhang, Peng and Li, Juying and Yang, Bo and Chen, Yantao and Liang, Li
Journal: International journal of biological macromolecules (2020): 814-822
Antibody-biotin-streptavidin-horseradish peroxidase (HRP) sensor for rapid and ultra-sensitive detection of fumonisins.
Authors: Yang, Hualin and Zhang, Qi and Liu, Xiaolei and Yang, Yuying and Yang, Yong and Liu, Mingyuan and Li, Peiwu and Zhou, Yu
Journal: Food chemistry (2020): 126356
Authors: Yang, Hualin and Zhang, Qi and Liu, Xiaolei and Yang, Yuying and Yang, Yong and Liu, Mingyuan and Li, Peiwu and Zhou, Yu
Journal: Food chemistry (2020): 126356
An efficient methodology for the purification of date palm peroxidase: Stability comparison with horseradish peroxidase (HRP).
Authors: Saud Al-Bagmi, Moneera and Shahnawaz Khan, Mohd and Alhasan Ismael, Mohamad and Al-Senaidy, Abdulrahman M and Ben Bacha, Abir and Mabood Husain, Fohad and Alamery, Salman Freeh
Journal: Saudi journal of biological sciences (2019): 301-307
Authors: Saud Al-Bagmi, Moneera and Shahnawaz Khan, Mohd and Alhasan Ismael, Mohamad and Al-Senaidy, Abdulrahman M and Ben Bacha, Abir and Mabood Husain, Fohad and Alamery, Salman Freeh
Journal: Saudi journal of biological sciences (2019): 301-307
Sensitive sulfide ion detection by optofluidic catalytic laser using horseradish peroxidase (HRP) enzyme.
Authors: Gong, Chaoyang and Gong, Yuan and Khaing Oo, Maung Kyaw and Wu, Yu and Rao, Yunjiang and Tan, Xiaotian and Fan, Xudong
Journal: Biosensors & bioelectronics (2017): 351-357
Authors: Gong, Chaoyang and Gong, Yuan and Khaing Oo, Maung Kyaw and Wu, Yu and Rao, Yunjiang and Tan, Xiaotian and Fan, Xudong
Journal: Biosensors & bioelectronics (2017): 351-357
Ultrasensitive electrochemical immunosensor based on horseradish peroxidase (HRP)-loaded silica-poly(acrylic acid) brushes for protein biomarker detection.
Authors: Zhao, Yan and Zheng, Yiqun and Kong, Rongmei and Xia, Lian and Qu, Fengli
Journal: Biosensors & bioelectronics (2016): 383-8
Authors: Zhao, Yan and Zheng, Yiqun and Kong, Rongmei and Xia, Lian and Qu, Fengli
Journal: Biosensors & bioelectronics (2016): 383-8
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