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Hoechst 33342 amine

Hoechst 33342 amine is an excellent building block for developing site-specific nucleic acid probes. It can be readily conjugated to carbonyl-containing biomolecules (such as antibodies, peptides and oligos). Hoechst 33342 is a DNA-specific dye that binds to the minor groove of double-stranded DNA. It emits blue fluorescence when bound to DNA. Its excitation maximum is around 350 nm, and the emission maximum is approximately 460 nm. Its ability to readily penetrate live cell membranes makes it suitable for staining live cells. This property allows researchers to monitor nuclear morphology and DNA content in living cells. Hoechst 33342 is commonly used in fluorescence microscopy to visualize the nucleus of cells.

Spectrum

References

View all 50 references: Citation Explorer
Inhibitory Effects of Trans-Cinnamaldehyde Against Pseudomonas aeruginosa Biofilm Formation.
Authors: Song, Luyi and Yang, Hui and Meng, Xinru and Su, Ruiying and Cheng, Shuai and Wang, Haoran and Bai, Xiangyang and Guo, Du and Lü, Xin and Xia, Xiaodong and Shi, Chao
Journal: Foodborne pathogens and disease (2023): 47-58
Experimental and Molecular Docking Studies on the Interaction of a Water-Soluble Pd(II) Complex Containing β-Amino Alcohol with Calf Thymus DNA.
Authors: Shahabadi, Nahid and Ghaffari, Lida and Mardani, Zahra and Shiri, Farshad
Journal: Biological trace element research (2022): 1988-2000
Anticancer and Antimicrobial Evaluations on Alternative Reading Frame (ARF) Peptides and their Derivatives.
Authors: Wang, Yuxin and Patel, Kamal and Wu, Zimei and Sarojini, Vijayalekshmi
Journal: Protein and peptide letters (2022): 242-253
In Vitro Effects of Mitochondria-Targeted Antioxidants in a Small-Cell Carcinoma of the Ovary of Hypercalcemic Type and in Type 1 and Type 2 Endometrial Cancer.
Authors: Castelôa, Mariana and Moreira-Pinto, Beatriz and Benfeito, Sofia and Borges, Fernanda and Fonseca, Bruno M and Rebelo, Irene
Journal: Biomedicines (2022)
A novel pyrrole-imidazole polyamide targets Aurora kinase A and suppresses tumor growth in vivo.
Authors: Li, Meiqing and Lu, Danyi and Cheng, Yulian and Wu, Chunlei and Zhang, Jianchao and Shi, Wenli and Ding, Zhihao and Li, Yanyan and Cheng, Binghua and Lin, Xian and Shao, Ximing and Li, Hongchang and Fang, Lijing and Liu, Ke and Su, Wu
Journal: Biochemical and biophysical research communications (2021): 167-173
Page updated on November 19, 2024

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Catalog Number17671
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Physical properties

Molecular weight

495.63

Solvent

DMSO

Spectral properties

Excitation (nm)

352

Emission (nm)

454

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
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