Hoechst 33258 *CAS 23491-45-4*
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Physical properties
Molecular weight | 533.88 |
Solvent | Water |
Spectral properties
Extinction coefficient (cm -1 M -1) | 460001 |
Excitation (nm) | 352 |
Emission (nm) | 454 |
Quantum yield | 0.03401 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H340 |
Hazard symbol | T |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R68 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Alternative formats
Hoechst 33258 *20 mM solution in water* |
Overview | SDSProtocol |
See also: Cell Cycle Assays, Nucleus, DNA Methylation, Fluorescence Activated Cell Sorting (FACS), Hoechst DNA Stains for Live and Fixed Cells, Immunohistochemistry (IHC), Nucleic Acid Building Blocks
CAS 23491-45-4 | Molecular weight 533.88 | Extinction coefficient (cm -1 M -1) 460001 | Excitation (nm) 352 | Emission (nm) 454 | Quantum yield 0.03401 |
The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Two of these closely related bis-benzimides are commonly used: Hoechst 33258 and Hoechst 33342. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescence light around an emission maximum at 461 nm. The Hoechst stains may be used on live or fixed cells, and are often used as a substitute for another nucleic acid stain, DAPI. The key difference between them is that the additional ethyl group of Hoechst 33342 renders it more lipophilic, and thus more able to cross intact cell membranes. In some applications, Hoechst 33258 is significantly less permeant. These dyes can also be used to detect the contents of a sample DNA by plotting a standard emission-to-content curve.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Hoechst 33258 *CAS 23491-45-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 187.308 µL | 936.54 µL | 1.873 mL | 9.365 mL | 18.731 mL |
5 mM | 37.462 µL | 187.308 µL | 374.616 µL | 1.873 mL | 3.746 mL |
10 mM | 18.731 µL | 93.654 µL | 187.308 µL | 936.54 µL | 1.873 mL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Extinction coefficient (cm -1 M -1) | 460001 |
Excitation (nm) | 352 |
Emission (nm) | 454 |
Quantum yield | 0.03401 |
Product Family
Name | Excitation (nm) | Emission (nm) |
Hoechst 33342 *Ultrapure Grade* | 352 | 454 |
Hoechst 34580 *CAS 911004-45-0* | 371 | 438 |
Hoechst 34580 *20 mM solution in water* | 371 | 438 |
Hoechst 33342 *20 mM solution in water* | 352 | 454 |
Images
Citations
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Authors: Chang, Chi-Sen and Shen, Yuh-Chiang and Juan, Chi-Wen and Chang, Chia-Lin and Lin, Po-Kai
Journal: Current Topics in Nutraceutical Research (2022)
Authors: Chang, Chi-Sen and Shen, Yuh-Chiang and Juan, Chi-Wen and Chang, Chia-Lin and Lin, Po-Kai
Journal: Current Topics in Nutraceutical Research (2022)
3'-Oxo-tabernaelegantine A (OTNA) selectively relaxes pulmonary arteries by inhibiting AhR
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Journal: Phytomedicine (2021)
Authors: Long, Pei and Li, Yong and Wen, Qing and Huang, Maohua and Li, Songtao and Lin, Yuning and Huang, Xiaojn and chen, Minfeng and Ouyang, Jie and Ao, Yunlin and others,
Journal: Phytomedicine (2021)
Plasma proteomic analysis of autoimmune hepatitis in an improved AIH mouse model
Authors: Wang, Han and Yan, Wei and Feng, Zuohua and Gao, Yuan and Zhang, Liu and Feng, Xinxia and Tian, Dean
Journal: Journal of Translational Medicine (2020): 1--16
Authors: Wang, Han and Yan, Wei and Feng, Zuohua and Gao, Yuan and Zhang, Liu and Feng, Xinxia and Tian, Dean
Journal: Journal of Translational Medicine (2020): 1--16
References
View all 42 references: Citation Explorer
DNA sequence recognition by Hoechst 33258 conjugates of hairpin pyrrole/imidazole polyamides
Authors: Correa BJ, Canzio D, Kahane AL, Reddy PM, Bruice TC.
Journal: Bioorg Med Chem Lett (2006): 3745
Authors: Correa BJ, Canzio D, Kahane AL, Reddy PM, Bruice TC.
Journal: Bioorg Med Chem Lett (2006): 3745
Recognition of B-DNA by neomycin--Hoechst 33258 conjugates
Authors: Willis B, Arya DP.
Journal: Biochemistry (2006): 10217
Authors: Willis B, Arya DP.
Journal: Biochemistry (2006): 10217
Design of new bidentate ligands constructed of two Hoechst 33258 units for discrimination of the length of two A3T3 binding motifs
Authors: Tanada M, Tsujita S, Sasaki S.
Journal: J Org Chem (2006): 125
Authors: Tanada M, Tsujita S, Sasaki S.
Journal: J Org Chem (2006): 125
The Hoechst 33258 Covalent Dimer Covers a Total Turn of the Double-stranded DNA
Authors: Streltsov SA, Gromyko AV, Oleinikov VA, Zhuze AL.
Journal: J Biomol Struct Dyn (2006): 285
Authors: Streltsov SA, Gromyko AV, Oleinikov VA, Zhuze AL.
Journal: J Biomol Struct Dyn (2006): 285
Amine terminated G-6 PAMAM dendrimer and its interaction with DNA probed by Hoechst 33258
Authors: Choi YS, Cho TS, Kim JM, Han SW, Kim SK.
Journal: Biophys Chem (2006): 142
Authors: Choi YS, Cho TS, Kim JM, Han SW, Kim SK.
Journal: Biophys Chem (2006): 142
Unique cooperative binding interaction observed between a minor groove binding Pt antitumor agent and Hoechst dye 33258
Authors: Harris A, Qu Y, Farrell N.
Journal: Inorg Chem (2005): 1196
Authors: Harris A, Qu Y, Farrell N.
Journal: Inorg Chem (2005): 1196
Hoechst 33258 as a pH-sensitive probe to study the interaction of amine oxide surfactants with DNA
Authors: Goracci L, Germani R, Savelli G, Bassani DM.
Journal: Chembiochem (2005): 197
Authors: Goracci L, Germani R, Savelli G, Bassani DM.
Journal: Chembiochem (2005): 197
Recognition of a 10 base pair sequence of DNA and stereochemical control of the binding affinity of chiral hairpin polyamide-Hoechst 33258 conjugates
Authors: Reddy PM, Toporowski JW, Kahane AL, Bruice TC.
Journal: Bioorg Med Chem Lett (2005): 5531
Authors: Reddy PM, Toporowski JW, Kahane AL, Bruice TC.
Journal: Bioorg Med Chem Lett (2005): 5531
Hydration changes in the association of Hoechst 33258 with DNA
Authors: Kiser JR, Monk RW, Smalls RL, Petty JT.
Journal: Biochemistry (2005): 16988
Authors: Kiser JR, Monk RW, Smalls RL, Petty JT.
Journal: Biochemistry (2005): 16988
Activity of Hoechst 33258 against Pneumocystis carinii f. sp. muris, Candida albicans, and Candida dubliniensis
Authors: Disney MD, Stephenson R, Wright TW, Haidaris CG, Turner DH, Gigliotti F.
Journal: Antimicrob Agents Chemother (2005): 1326
Authors: Disney MD, Stephenson R, Wright TW, Haidaris CG, Turner DH, Gigliotti F.
Journal: Antimicrob Agents Chemother (2005): 1326
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides