Calculator
Hexidium Iodide *CAS 211566-66-4*
The nucleic acid stain, hexidium iodide is permeant to mammalian cells and selectively stains almost all gram-positive bacteria in the presence of gram-negative bacteria. Hexidium iodide exhibits excitation/emission maxima of ~518/600 nm upon binding DNA. Generally, both the cytoplasm and nuclei of eukaryotic cells show staining with hexidium iodide. We do not recommend its use as a nuclear stain since it also stains other subcelluar compartments.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Hexidium Iodide *CAS 211566-66-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 201.041 µL | 1.005 mL | 2.01 mL | 10.052 mL | 20.104 mL |
| 5 mM | 40.208 µL | 201.041 µL | 402.083 µL | 2.01 mL | 4.021 mL |
| 10 mM | 20.104 µL | 100.521 µL | 201.041 µL | 1.005 mL | 2.01 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
| Propidium iodide *CAS 25535-16-4* | 537 | 618 | 60001 | 0.21 |
| Propidium iodide *10 mM aqueous solution* | 537 | 618 | 60001 | 0.21 |
| DiR iodide [1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide] | 754 | 778 | 2700001 | - |
| DiI iodide [1,1-Dioctadecyl-3,3,3,3- tetramethylindocarbocyanine iodide] | 550 | 564 | 148000 | - |
| Propidium iodide *1 mg/mL aqueous solution* | 537 | 618 | 60001 | 0.21 |
Citations
View all 1 citations: Citation Explorer
Functional and Quantitative High-Throughput Screening for Hydrolases Secreted by B. subtilis
Authors: Dabene, Valentina
Journal: (2021)
Authors: Dabene, Valentina
Journal: (2021)
References
View all 13 references: Citation Explorer
Novel model for multispecies biofilms that uses rigid gas-permeable lenses
Authors: Peyyala R, Kirakodu SS, Ebersole JL, Novak KF.
Journal: Appl Environ Microbiol (2011): 3413
Authors: Peyyala R, Kirakodu SS, Ebersole JL, Novak KF.
Journal: Appl Environ Microbiol (2011): 3413
Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum
Authors: Linhova M, Patakova P, Lipovsky J, Fribert P, Paulova L, Rychtera M, Melzoch K.
Journal: Folia Microbiol (Praha) (2010): 340
Authors: Linhova M, Patakova P, Lipovsky J, Fribert P, Paulova L, Rychtera M, Melzoch K.
Journal: Folia Microbiol (Praha) (2010): 340
A flow cytometric technique for quantification and differentiation of bacteria in bulk tank milk
Authors: Holm C, Mathiasen T, Jespersen L.
Journal: J Appl Microbiol (2004): 935
Authors: Holm C, Mathiasen T, Jespersen L.
Journal: J Appl Microbiol (2004): 935
Metabolic activity in filamentous fungi can be analysed by flow cytometry
Authors: Bradner JR, Nevalainen KM.
Journal: J Microbiol Methods (2003): 193
Authors: Bradner JR, Nevalainen KM.
Journal: J Microbiol Methods (2003): 193
Potential for broad applications of flow cytometry and fluorescence techniques in microbiological and somatic cell analyses of milk
Authors: Gunasekera TS, Veal DA, Attfield PV.
Journal: Int J Food Microbiol (2003): 269
Authors: Gunasekera TS, Veal DA, Attfield PV.
Journal: Int J Food Microbiol (2003): 269
Page updated on November 21, 2024
Safety data sheet