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Helixyte™ Green NHS ester
Helixyte™ Green is an excellent nucleic acid sensor that exhibits large fluorescence enhancement upon binding to dsDNA. It has the same spectral and DNA-binding properties as SYBR Green® (SYBR Green® is the trademark of ThermoFisher). AAT Bioquest’s scientists developed Helixyte™ Green NHS ester to enable the development of site-specific DNA probes. Helixyte™ Green NHS ester readily reacts with amino-containing biological molecules such as peptides, antibodies and amino-modified oligos. Thus it can be used to develop a variety of site-directed DNA probes. Helixyte™ Green is an excellent fluorogenic probe for quantifying DNAs with greatly improved sensitivity and selectivity. Helixyte™ Green is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biological procedures such as cDNA synthesis for library production and DNA fragment purification for subcloning, as well as diagnostic applications, such as quantitating DNA amplification products and primer extension assays. Using the Helixyte™ Green dsDNA Quantifying Reagent, you can selectively detect as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides.
Spectrum
References
View all 50 references: Citation Explorer
Rapid determination of antibiotic susceptibility of clinical isolates of Escherichia coli by SYBR green I/Propidium iodide assay.
Authors: Cui, Xianglun and Liu, Shuyue and Jin, Yan and Li, Mingyu and Shao, Chunhong and Yu, Hong and Zhang, Ying and Liu, Yun and Wang, Yong
Journal: Scientific reports (2024): 18782
Authors: Cui, Xianglun and Liu, Shuyue and Jin, Yan and Li, Mingyu and Shao, Chunhong and Yu, Hong and Zhang, Ying and Liu, Yun and Wang, Yong
Journal: Scientific reports (2024): 18782
First Report on the Molecular Detection of Canine Astrovirus (CaAstV) in Dogs with Gastrointestinal Disease in Ecuador Using a Fast and Sensitive RT-qPCR Assay Based on SYBR Green®.
Authors: Loor-Giler, Anthony and Castillo-Reyes, Sara and Santander-Parra, Silvana and Campos, Martín and Mena-Pérez, Renán and Prado-Chiriboga, Santiago and Nuñez, Luis
Journal: Veterinary sciences (2024)
Authors: Loor-Giler, Anthony and Castillo-Reyes, Sara and Santander-Parra, Silvana and Campos, Martín and Mena-Pérez, Renán and Prado-Chiriboga, Santiago and Nuñez, Luis
Journal: Veterinary sciences (2024)
Detection of SARS-CoV-2 B.1.1.529 (Omicron) variant by SYBR Green-based RT-qPCR.
Authors: Abdel-Sater, Fadi and Makki, Rawan and Khalil, Alia and Hussein, Nader and Borghol, Nada and Abi Khattar, Ziad and Hamade, Aline and Khreich, Nathalie and El Homsi, Mahoumd and Kanaan, Hussein and Raad, Line and Skafi, Najwa and Al-Nemer, Fatima and Ghandour, Zeinab and El-Zein, Nabil and Abou-Hamdan, Mhamad and Akl, Haidar and Hamade, Eva and Badran, Bassam and Hamze, Kassem
Journal: Biology methods & protocols (2024): bpae020
Authors: Abdel-Sater, Fadi and Makki, Rawan and Khalil, Alia and Hussein, Nader and Borghol, Nada and Abi Khattar, Ziad and Hamade, Aline and Khreich, Nathalie and El Homsi, Mahoumd and Kanaan, Hussein and Raad, Line and Skafi, Najwa and Al-Nemer, Fatima and Ghandour, Zeinab and El-Zein, Nabil and Abou-Hamdan, Mhamad and Akl, Haidar and Hamade, Eva and Badran, Bassam and Hamze, Kassem
Journal: Biology methods & protocols (2024): bpae020
Development of a fast and sensitive RT-qPCR assay based on SYBR® green for diagnostic and quantification of Avian Nephritis Virus (ANV) in chickens affected with enteric disease.
Authors: Loor-Giler, Anthony and Castillo-Reyes, Sara and Santander-Parra, Silvana and Caza, Manuel and Kyriakidis, Nikolaos C and Ferreira, Antonio J Piantino and Nuñez, Luis
Journal: BMC veterinary research (2024): 33
Authors: Loor-Giler, Anthony and Castillo-Reyes, Sara and Santander-Parra, Silvana and Caza, Manuel and Kyriakidis, Nikolaos C and Ferreira, Antonio J Piantino and Nuñez, Luis
Journal: BMC veterinary research (2024): 33
A label free fluorescent aptamer sensor based on the combined action of Graphene oxide and SYBR Green I for the detection of Aflatoxin B1.
Authors: Hu, Mengyang and Cheng, Meng and Wang, Na and Sang, Yidan and Dong, Yafei and Wang, Luhui
Journal: IEEE transactions on nanobioscience (2024)
Authors: Hu, Mengyang and Cheng, Meng and Wang, Na and Sang, Yidan and Dong, Yafei and Wang, Luhui
Journal: IEEE transactions on nanobioscience (2024)
Page updated on February 12, 2025
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