Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *Optimized for Broad Dynamic Range*

Helixyte™ Green Fluorimetric dsDNA Quantitation Kit

Optimized for Broad Dynamic Range

DNA Quantitation is a very important task in DNA sample preparations for various analyses. Helixyte™ Green Fluorimetric dsDNA Quantitation Kit provides a rapid method to quantify dsDNA with Helixyte™ Green BR . The assay is linear over three orders of magnitude and is a few magnitudes more sensitive than UV absorbance readings. Helixyte™ Green BR exhibits large fluorescence enhancement upon binding to dsDNA and has little sequence dependence, allowing to the accurate measurement of DNA samples from various sources, including genomic DNA, viral DNA, miniprep DNA or PCR amplification products. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is optimized to measure DNA concentrations from 10 pg/µL to 10 ng/µL.

dsDNA dose response was measured with Helixyte™ Green Fluorimetric dsDNA Quantitation Kit in a 96-well solid black plate.

Protocol

COA

Catalog	Size	Price	Quantity
17645	200 Tests	Price
17646	1000 Tests	Price

Citations

View all 34 citations: Citation Explorer

Enhanced articular cartilage regeneration using costal chondrocyte-derived scaffold-free tissue engineered constructs with ascorbic acid treatment

Authors:

Zheng, Kaiwen and Ma, Yiyang and Chiu, Cheng and Xue, Mengxin and Zhang, Changqing and Du, Dajiang

Journal:

Journal of Orthopaedic Translation (2024): 140--154

The Response of the Soil Microbiota to Long-Term Mineral and Organic Nitrogen Fertilization is Stronger in the Bulk Soil than in the Rhizosphere

Authors:

Cardinale, Massimiliano and Ratering, Stefan and Sadeghi, Aitak and Pokhrel, Sushil and Honermeier, Bernd and Schnell, Sylvia

Journal:

Genes (2020): 456

Quantification of fixed adherent cells using a strong enhancer of the fluorescence of DNA dyes

Authors:

Ligasova, A., Koberna, K.

Journal:

Sci Rep (2019): 8701

Molecular-Recognition-Based DNA Nanodevices for Enhancing the Direct Visualization and Quantification of Single Vesicles of Tumor Exosomes in Plasma Microsamples

Authors:

He, D., Ho, S. L., Chan, H. N., Wang, H., Hai, L., He, X., Wang, K., Li, H. W.

Journal:

Anal Chem (2019): 2768-2775

A universal fluorescence-based toolkit for real-time quantification of DNA and RNA nuclease activity

Authors:

Sheppard, E. C., Rogers, S., Harmer, N. J., Chahwan, R.

Journal:

Sci Rep (2019): 8853

Spectral properties

Excitation (nm)	503
Emission (nm)	527

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	530 nm
Cutoff	515 nm
Recommended plate	Solid black

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Page updated on October 13, 2025

Component A: Helixyte™ Green BR	1 vial (0.1 mL-200X in DMSO)
Component B: Assay Buffer	1 bottle (50 mL)
Component C: DNA Standard	1 vial (1 mL, Calf thymus DNA: 100 ug/mL)

Component A: Helixyte™ Green BR	1 vial (0.5 mL, 200X in DMSO)
Component B: Assay Buffer	3 bottles (85 mL/bottle)
Component C: DNA Standard	1 vial (5 mL, Calf thymus DNA: 100 ug/mL)