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Glucose-UDP-Fluorescein Conjugate
Uridine-5'-diphospho-1-alpha-D-glucose (UDP-Glc) is a key intermediate in carbohydrate metabolism. UDP-Glc serves as a precursor of glycogen, can be metabolized into UDP-galactose and UDP-glucuronic acid which can then be incorporated into polysaccharides as galactose and glucuronic acid. UDP-Glc serves as a precursor of sucrose lipopolysaccharides and glycosphingolipids. UDP-Glc is a common substrate used by glucosyltransferases, including certain bacterial toxins such as Toxins A and B from Clostridium difficile. Fluorescent analogs of UDP-Glc might be used for studying glucosyltransferases and clostridial toxins. This fluorescein UDP-Glc derivative has the spectral properties identical to those of FITC conjugates.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Glucose-UDP-Fluorescein Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 77.927 µL | 389.636 µL | 779.271 µL | 3.896 mL | 7.793 mL |
| 5 mM | 15.585 µL | 77.927 µL | 155.854 µL | 779.271 µL | 1.559 mL |
| 10 mM | 7.793 µL | 38.964 µL | 77.927 µL | 389.636 µL | 779.271 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
References
View all 161 references: Citation Explorer
cDNA cloning and characterization of UDP-glucose: anthocyanidin 3-O-glucosyltransferase in Freesia hybrida
Authors: Sui X, Gao X, Ao M, Wang Q, Yang D, Wang M, Fu Y, Wang L.
Journal: Plant Cell Rep (2011): 1209
Authors: Sui X, Gao X, Ao M, Wang Q, Yang D, Wang M, Fu Y, Wang L.
Journal: Plant Cell Rep (2011): 1209
Downregulation of putative UDP-glucose: flavonoid 3-O-glucosyltransferase gene alters flower coloring in Phalaenopsis
Authors: Chen WH, Hsu CY, Cheng HY, Chang H, Chen HH, Ger MJ.
Journal: Plant Cell Rep (2011): 1007
Authors: Chen WH, Hsu CY, Cheng HY, Chang H, Chen HH, Ger MJ.
Journal: Plant Cell Rep (2011): 1007
Molecular analysis of a UDP-glucose: flavonoid 3-O-glucosyltransferase (UFGT) gene from purple potato (Solanum tuberosum)
Authors: Hu C, Gong Y, Jin S, Zhu Q.
Journal: Mol Biol Rep (2011): 561
Authors: Hu C, Gong Y, Jin S, Zhu Q.
Journal: Mol Biol Rep (2011): 561
Identification of a UDP-glucose pyrophosphorylase from cotton (Gossypium hirsutum L.) involved in cellulose biosynthesis in Arabidopsis thaliana
Authors: Wang Q, Zhang X, Li F, Hou Y, Liu X.
Journal: Plant Cell Rep (2011): 1303
Authors: Wang Q, Zhang X, Li F, Hou Y, Liu X.
Journal: Plant Cell Rep (2011): 1303
Molecular cloning and analysis of the UDP-Glucose Pyrophosphorylase in Streptococcus equi subsp. zooepidemicus
Authors: Ma Z, Fan HJ, Lu CP.
Journal: Mol Biol Rep (2011): 2751
Authors: Ma Z, Fan HJ, Lu CP.
Journal: Mol Biol Rep (2011): 2751
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