Gelite™ Safe DNA Gel Stain *10,000X DMSO Solution*
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Catalog Number | |
Unit Size | |
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Molecular weight | N/A |
Solvent | Water |
Absorbance (nm) | 509 |
Excitation (nm) | 513 |
Emission (nm) | 552 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
Molecular weight N/A | Absorbance (nm) 509 | Excitation (nm) 513 | Emission (nm) 552 |
Platform
Gel Imager
Excitation | UV Transilluminator/Blue laser |
Emission | SYBR® filter, GelStar® filter, GelGreen® filter, or GelRed® filter |
Example protocol
PREPARATION OF WORKING SOLUTION
Make 1X Gelite™ Safe working solution by diluting the 10,000X stock reagent with a buffer of your choice in a pH range of 7.5-8.5 (e.g., TAE, TBE, or TE, preferably pH 8.2).
Note: Staining solutions prepared in water are less stable than those prepared in buffer and must be used within 24 hours to ensure maximal staining sensitivity.
SAMPLE EXPERIMENTAL PROTOCOL
The following protocols are recommended. However, some comparisons might be made to determine which one better meets your needs.
Run gels according to your standard protocol.
Place the gel in a suitable polypropylene container. Gently add a sufficient amount of the 1X staining solution to submerge the gel.
Note: Do not use a glass container, as it will adsorb much of the dye in the staining solution.
Agitate the gel gently at room temperature for ~30 to 60 minutes. Protect the staining container from light.
Note: Destaining is not required. Image can be acquired without any wash steps.
- Image the gel with a 300 nm/254 nm ultraviolet transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter, GelStar® filter, GelGreen® filter, or GelRed® filter.
Prepare agarose gel solution using your standard protocol.
Dilute the 10,000X Gelite™ Safe stock reagent into the gel solution at 1:10,000 just prior to pouring the gel and mix thoroughly.
Run gels according to your standard protocol.
Image the gel with a 300 nm/254 nm ultraviolet transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter, GelStar® filter, GelGreen® filter, or GelRed® filter.
Spectrum
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fgelite_safe_dna_gel_stain.png&w=2048&q=50)
Spectral properties
Absorbance (nm) | 509 |
Excitation (nm) | 513 |
Emission (nm) | 552 |
Images
![<strong>Comparison of DNA detection in 1% agarose gel in TBE buffer using Gelite™ Safe, EtBr, and SYBR® Safe.</strong> Two-fold serial dilutions of 1 kb DNA ladder were loaded in amounts of 100 ng, 50 ng, and 25 ng from left to right. Gels were stained for 60 minutes with Gelite™ Safe, EtBr, and SYBR® Safe according to the manufacturer's recommended concentrations and imaged using the ChemiDoc™ Imaging System (Bio-Rad®). Gels were illuminated using a 300 nm transilluminator fitted with a GelGreen filter.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fgelite-safe-dna-gel-stain-10-000x-dmso-solution%2Ffigure-for-gelite-safe-dna-gel-stain-10-000x-dmso-solution_p2pvj.jpg&w=3840&q=75)
![Comparison of Gelite™ Safe (1:25,000X dilution) and GelRed® (1:10,000X dilution) in precast gel staining using 1% agarose gel in TBE buffer. Two-fold serial dilutions of 1 kb DNA ladder were loaded in the amounts of 100 ng, 50 ng, 25 ng, 12 ng, 6 ng, 3 ng, 1.5 ng, and 0.7 ng from left to right. Gels were imaged using a 300 nm transilluminator in ChemiDoc™ Imaging System (Bio-Rad®).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fgelite-safe-dna-gel-stain-10-000x-dmso-solution%2Ffigure-for-gelite-safe-dna-gel-stain-10-000x-dmso-solution_6gaHG.png&w=3840&q=75)
![Stability test on Gelite™ Safe DNA Gel Stain, which was dissolved in DMSO and stored at four different temperatures (-20°C, 4°C, room temperature, and 37°C) for a period of one month. After storage, DNA ladders (100, 50, and 25 ng) were loaded in 1% agarose gel for 60 minutes at 75 V. Gels were incubated with Gelite™ Safe DNA Gel Stain for 1 hour. The image was captured in SYBR green filter set (Exposure time= 1.5 sec).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fgelite-safe-dna-gel-stain-10-000x-dmso-solution%2Ffigure-for-gelite-safe-dna-gel-stain-10-000x-dmso-solution_AIKke.png&w=3840&q=75)
![Stability test on Gelite™ Safe DNA Gel Stain, which was dissolved in DMSO and stored at four different temperatures (-20°C, 4°C, room temperature, and 37°C) for a period of three months. After storage, DNA ladders (100, 50, and 25 ng) were loaded in 1% agarose gel for 60 minutes at 75 V. Gels were incubated with Gelite™ Safe DNA Gel Stain for 1 hour. The image was captured in SYBR green filter set (Exposure time= 1.5 sec).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fgelite-safe-dna-gel-stain-10-000x-dmso-solution%2Ffigure-for-gelite-safe-dna-gel-stain-10-000x-dmso-solution_q1ENl.png&w=3840&q=75)
![Comparison of DNA detection in 1% agarose gel in TBE buffer using Gelite™ Safe and GelRed®. Two-fold serial dilutions of 1 kb DNA ladder were loaded in amounts of 100 ng, 50 ng, and 25 ng from left to right. Gels were stained for 60 minutes with Gelite™ Safe and GelRed™ (Biotium) according to the manufacturer's recommended concentrations and imaged using the ChemiDoc™ Imaging System (Bio-Rad®). Gels were illuminated using a 300 nm transilluminator fitted with an EtBr filter set.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fgelite-safe-dna-gel-stain-10-000x-dmso-solution%2Ffigure-for-gelite-safe-dna-gel-stain-10-000x-dmso-solution_HWV9d.png&w=3840&q=75)
![Comparison of DNA detection in 1% agarose gel in TBE buffer using Gelite™ Safe, SYBR® Safe, and EtBr. Two-fold serial dilutions of 1 kb DNA ladder were loaded in amounts of 86 ng, 43 ng, 21.5 ng, 10.7 ng, 5.3 ng, 2.6 ng, 1.3 ng, and 0.5 ng from left to right. Gels were imaged using a 300 nm transilluminator in ChemiDoc™ Imaging System (Bio-Rad®).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fgelite-x100-dna-gel-stain-10-000x-dmso-solution%2Ffigure-for-gelite-x100-dna-gel-stain-10-000x-dmso-solution_E0I9R.jpg&w=3840&q=75)
![Summary of Ames test results. Ames mutagenicity test was performed in a dose-dependent manner for Gelite™ Safe, SYBR® Green, and EtBr. Samples were pretreated with an S9 fraction liver extract and then tested. With <em>S. Typhimurium</em> strain TA1538, an increase in revertants of more than two-fold over the background indicates a positive result for mutagenicity.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fgelite-x100-dna-gel-stain-10-000x-dmso-solution%2Ffigure-for-gelite-x100-dna-gel-stain-10-000x-dmso-solution_Kkwig.jpg&w=3840&q=75)
Citations
Authors: Esquivel, Maria and Fernando, Johann and Fisher, Anna and Leong, Cameron and Weaver, Adam
Journal: (2022)
References
Authors: Zin, Nik Nor Imam Nik Mat and Mohamad, Mira Nabila and Roslan, Keusar and Abdul Wafi, Sazeli and Abdul Moin, Nurul I'zaaz and Alias, Azamuddin and Zakaria, Yusmazura and Abu-Bakar, Nurhidanatasha
Journal: The Malaysian journal of medical sciences : MJMS (2020): 36-50
Authors: Gopinathan, Adarsh and Moidu, Mahreen and Mukundan, Minil and Ellickal Narayanan, Siju and Narayanan, Hariraj and Adhikari, Navin
Journal: Drug development research (2020)
Authors: Won, Joungha and Lee, Solji and Park, Myungsun and Kim, Tai Young and Park, Mingu Gordon and Choi, Byung Yoon and Kim, Dongwan and Chang, Hyeshik and Kim, V Narry and Lee, C Justin
Journal: Experimental neurobiology (2020): 107-119
Authors: Domiati, S and Mehanna, M and Ragab, H and El Galil, K H Abd and Nakkash-Chmaisse, H and El Mallah, A
Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.] (2019): 379-386
Authors: Thita, T and Manomat, J and Leelayoova, S and Mungthin, M and Ruang-Areerate, T
Journal: Tropical biomedicine (2019): 495-504
Authors: Motohashi, Ken
Journal: PloS one (2019): e0222209
Authors: O'Neil, Casey S and Beach, Jacie L and Gruber, Todd D
Journal: Electrophoresis (2018): 1474-1477
Authors: Kwong, K M and Tam, C C and Chan, Raymond and Lee, Stephen W L and Ip, P and Kwok, Janette
Journal: Clinica chimica acta; international journal of clinical chemistry (2018): 45-50
Authors: Hou, Rui and Li, Muzi and Tang, Tingting and Wang, Ruichong and Li, Yijing and Xu, Yigang and Tang, Lijie and Wang, Li and Liu, Min and Jiang, Yanping and Cui, Wen and Qiao, Xinyuan
Journal: BMC microbiology (2018): 80
Authors: Bernemann, Christof and Steinestel, Julie and Humberg, Verena and Bögemann, Martin and Schrader, Andres Jan and Lennerz, Jochen K
Journal: BJU international (2018): 219-226
Application notes
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