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Gelite™ Red Nucleic Acid Gel Stain *Replaced by #17707*

Gelite™ Red is the most recent addition to our Gelite™ nucleic acid gel stain family. Now we have a complete family of multicolor gel stains for detecting nucleic acids in gels. Gelite™ Red is an extremely sensitive nucleic acid gel stain for detecting DNA in gels using a standard 300 nm UV transilluminator and Polaroid 667 black-and-white print film. Under the same conditions it is more sensitive than the popular SYBR® Gold gel stain. This remarkable sensitivity can be attributed to a combination of unique dye characteristics of Gelite™ Red. Because the nucleic acid"bound Gelite™ Red dye exhibits excitation maxima close to 488 nm and ~300 nm (the emission maximum is ~610 nm), it is compatible with a wide variety of instrumentation, ranging from UV epi- and transilluminators and blue-light transilluminators, to mercury-arc lamp" and argon-ion laser"based gel scanners. Our Gelite™ Red is a superior alternative to SYBR® Gold, SYBR® Safe and Ethidium Bromide. It provides a convenient solution for staining nucleic acid samples in gels.

Citations

View all 14 citations: Citation Explorer
Ethidium DNA agarose gel electrophoresis: how it started
Authors: Borst, P.
Journal: IUBMB Life (2005): 745-7
Comparison of ethidium bromide-stained agarose gel electrophoresis and automated fragment analysis for evaluation of IgH gene products
Authors: Gleissner, B., Maurer, J., Sindram, A., Reinhard, R., Thiel, E.
Journal: Leuk Res (2001): 769-74
An enzymatic procedure for the purification of DNA restriction fragments without gel electrophoresis and ethidium bromide staining
Authors: Ratel, D., Dupre, I., Allamane, S., Berger, F., Verna, J. M., Benabid, A. L., Wion, D.
Journal: C R Acad Sci III (2000): 753-6
Comparison of SYBR Green I nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in the Salmonella/mammalian microsome reverse mutation assay (Ames test)
Authors: Singer, V. L., Lawlor, T. E., Yue, S.
Journal: Mutat Res (1999): 37-47
Effects of ethidium bromide on DNA loop organisation in human lymphocytes measured by anomalous viscosity time dependence and single cell gel electrophoresis
Authors: Belyaev, I. Y., Eriksson, S., Nygren, J., Torudd, J., Harms-Ringdahl, M.
Journal: Biochim Biophys Acta (1999): 348-56
Page updated on February 1, 2023

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Catalog Number17603
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Storage, safety and handling

H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC41116134
Comparison of ethidium bromide (EtBr) and Gelite&trade; Red in precast gel staining using 1% agarose gel in TBE buffer. 100 ng, 50 ng, 25 ng 1 kb Plus DNA Ladder (Invitrogen) were loaded from left to right. Gels were imaged using ChemiDoc&trade; MP Imager with an EtBr filter.
Comparison of ethidium bromide (EtBr) and Gelite&trade; Red in precast gel staining using 1% agarose gel in TBE buffer. 100 ng, 50 ng, 25 ng 1 kb Plus DNA Ladder (Invitrogen) were loaded from left to right. Gels were imaged using ChemiDoc&trade; MP Imager with an EtBr filter.
Comparison of ethidium bromide (EtBr) and Gelite&trade; Red in precast gel staining using 1% agarose gel in TBE buffer. 100 ng, 50 ng, 25 ng 1 kb Plus DNA Ladder (Invitrogen) were loaded from left to right. Gels were imaged using ChemiDoc&trade; MP Imager with an EtBr filter.
Comparison of Gelite&trade; Red and SYBR&trade; Gold&nbsp; in post&nbsp; gel staining. 1 kb Plus DNA Ladder (200ng and 100ng per lane ) were subjected to electrophoresis in 0.9% agarose in 1 x TAE buffer. The gels were subsequently stained with a&nbsp;1x Gelite&trade; Red or SYBR&trade; Gold in 1 x TAE for 30 minutes and photographed using ChemiDoc&trade; MP Imager with an EtBr filter.