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Fura Red, potassium salt
Fura Red is a visible light-excitable fura-2 analog that offers unique possibilities for ratiometric measurement of calcium ion in single cells by microphotometry, imaging or flow cytometry when used with single excitation, green-fluorescent calcium indicators. Fura Red AM is the cell-permeable version of Fura Red used for noninvasive intracellular loading. Fura Red AM can be simultaneously loaded into cells with Fluo-3 AM, Fluo-8 AM or Cal-520 AM. An advantage of combining two calcium dyes is that dyes with longer excitation wavelengths can be used. This usually causes less harm to the cells than using ratiometric dyes that are excited with UV- or near UV-light (e.g. Fura-2), as light at visible wavelengths is less phototoxic.
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Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Fura Red, potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 123.612 µL | 618.062 µL | 1.236 mL | 6.181 mL | 12.361 mL |
| 5 mM | 24.722 µL | 123.612 µL | 247.225 µL | 1.236 mL | 2.472 mL |
| 10 mM | 12.361 µL | 61.806 µL | 123.612 µL | 618.062 µL | 1.236 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| Fura-10™, potassium salt | 354 | 524 |
References
View all 12 references: Citation Explorer
Ratiometric analysis of fura red by flow cytometry: a technique for monitoring intracellular calcium flux in primary cell subsets
Authors: Wendt ER, Ferry H, Greaves DR, Keshav S.
Journal: PLoS One (2015): e0119532
Authors: Wendt ER, Ferry H, Greaves DR, Keshav S.
Journal: PLoS One (2015): e0119532
A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux
Authors: Bailey S, Macardle PJ.
Journal: J Immunol Methods (2006): 220
Authors: Bailey S, Macardle PJ.
Journal: J Immunol Methods (2006): 220
Use of co-loaded Fluo-3 and Fura Red fluorescent indicators for studying the cytosolic Ca(2+)concentrations distribution in living plant tissue
Authors: Walczysko P, Wagner E, Albrechtova JT.
Journal: Cell Calcium (2000): 23
Authors: Walczysko P, Wagner E, Albrechtova JT.
Journal: Cell Calcium (2000): 23
Monitoring calcium in outer hair cells with confocal microscopy and fluorescence ratios of fluo-3 and fura-red
Authors: Su ZL, Li N, Sun YR, Yang J, Wang IM, Jiang SC.
Journal: Shi Yan Sheng Wu Xue Bao (1998): 323
Authors: Su ZL, Li N, Sun YR, Yang J, Wang IM, Jiang SC.
Journal: Shi Yan Sheng Wu Xue Bao (1998): 323
Calcium transient alternans in blood-perfused ischemic hearts: observations with fluorescent indicator fura red
Authors: Wu Y, Clusin WT.
Journal: Am J Physiol (1997): H2161
Authors: Wu Y, Clusin WT.
Journal: Am J Physiol (1997): H2161
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