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FluoroQuest™ Fluorescence Signal Enhancing Solution
When proteins are conjugated to fluorescent organic dyes, fluorescence emission of the dye molecules is usually decreased, sometimes up to 50-70%. This quenching phenomenon has been acknowledged for decades, but as yet, there are no simple, practical methods to control the fluorescence of dyes conjugated to proteins, especially for dyes conjugated to immunoglobulins. We offer this FluoroQuest™ fluorescence signal enhancing solution that might increase fluorescence up to 2.5-fold in cell imaging and flow analysis. This ready-to-use solution provides an effective way to increase the sensitivity of detection of fluorescent organic labels used in immunology, histochemistry, and cell biology.
References
View all 18 references: Citation Explorer
On the mechanism of Trolox as antiblinking and antibleaching reagent
Authors: Cordes T, Vogelsang J, Tinnefeld P.
Journal: J Am Chem Soc (2009): 5018
Authors: Cordes T, Vogelsang J, Tinnefeld P.
Journal: J Am Chem Soc (2009): 5018
Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification
Authors: Gallardo-Escarate C, Alvarez-Borrego J, Von Br and E, Dupre E, Del Rio-Portilla MA.
Journal: Biol Res (2007): 29
Authors: Gallardo-Escarate C, Alvarez-Borrego J, Von Br and E, Dupre E, Del Rio-Portilla MA.
Journal: Biol Res (2007): 29
Strategies to improve photostabilities in ultrasensitive fluorescence spectroscopy
Authors: Widengren J, Chmyrov A, Eggeling C, Lofdahl PA, Seidel CA.
Journal: J Phys Chem A (2007): 429
Authors: Widengren J, Chmyrov A, Eggeling C, Lofdahl PA, Seidel CA.
Journal: J Phys Chem A (2007): 429
The reliability of long-term storage of direct immunofluorescent staining slides at room temperature
Authors: Dikicioglu E, Meteoglu I, Okyay P, Culhaci N, Kacar F.
Journal: J Cutan Pathol (2003): 430
Authors: Dikicioglu E, Meteoglu I, Okyay P, Culhaci N, Kacar F.
Journal: J Cutan Pathol (2003): 430
Comparative genomic hybridization technique
Authors: El-Rifai WE, Knuutila S.
Journal: Methods Mol Med (2001): 25
Authors: El-Rifai WE, Knuutila S.
Journal: Methods Mol Med (2001): 25
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