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FDG [Fluorescein di-beta-D-galactopyranoside] *CAS#: 17817-20-8*
Although chromogenic assays of beta-galactosidase activity (i.e. X-Gal) are still widely used, the recent advance in fluorescence instrumentation has made fluorogenic substrates (such as FDG and MUG) be several orders of magnitude more sensitive. FDG is considered to be one of the most sensitive fluorogenic substrates available for detecting beta-galactosidase. The colorless and nonfluorescent FDG is hydrolyzed to highly fluorescent fluorescein, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments. Galactosidase-catalyzed hydrolysis of FDG can be followed by fluorescence increase around 520 nm. Alternatively, FDG can also be used to detect ?-galactosidase in a chromogenic mode since the enzymatic product (fluorescein) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). FDG has been widely used for identifying LacZ-positive cells with fluorescence microscopy and flow cytometry. It is also used to detect beta-galactosidase expression in live cells. Fluorescence-based assays employing FDG are also reported to be 100 to 1000-fold more sensitive than radioisotope-based ELISAs.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of FDG [Fluorescein di-beta-D-galactopyranoside] *CAS#: 17817-20-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 152.304 µL | 761.522 µL | 1.523 mL | 7.615 mL | 15.23 mL |
| 5 mM | 30.461 µL | 152.304 µL | 304.609 µL | 1.523 mL | 3.046 mL |
| 10 mM | 15.23 µL | 76.152 µL | 152.304 µL | 761.522 µL | 1.523 mL |
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Citations
View all 6 citations: Citation Explorer
Capturing nascent extracellular vesicles by metabolic glycan labeling-assisted microfluidics
Authors: Wu, Qiuyue and Wang, Wencheng and Zhang, Chi and You, Zhenlong and Zeng, Yinyan and Lu, Yinzhu and Zhang, Suhui and Li, Xingrui and Yang, Chaoyong and Song, Yanling
Journal: Nature Communications (2023): 6541
Authors: Wu, Qiuyue and Wang, Wencheng and Zhang, Chi and You, Zhenlong and Zeng, Yinyan and Lu, Yinzhu and Zhang, Suhui and Li, Xingrui and Yang, Chaoyong and Song, Yanling
Journal: Nature Communications (2023): 6541
Temporal Differentiation of Extracellular Vesicles by Metabolic Glycan Labeling-Assisted Microfluidics
Authors: Wu, Qiuyue and Wang, Wencheng and Zhang, Chi and You, Zhenlong and Zeng, Yinyan and Lu, Yinzhu and Zhang, Suhui and Li, Xingrui and Yang, Chaoyong and Song, Yanling
Journal: (2023)
Authors: Wu, Qiuyue and Wang, Wencheng and Zhang, Chi and You, Zhenlong and Zeng, Yinyan and Lu, Yinzhu and Zhang, Suhui and Li, Xingrui and Yang, Chaoyong and Song, Yanling
Journal: (2023)
Aptamer/antibody sandwich method for digital detection of SARS-CoV2 nucleocapsid protein
Authors: Ge, Chenchen and Feng, Juan and Zhang, Jiaming and Hu, Kai and Wang, Dou and Zha, Ling and Hu, Xuejuan and Li, Rongsong
Journal: Talanta (2021): 122847
Authors: Ge, Chenchen and Feng, Juan and Zhang, Jiaming and Hu, Kai and Wang, Dou and Zha, Ling and Hu, Xuejuan and Li, Rongsong
Journal: Talanta (2021): 122847
Gfi1b regulates the level of Wnt/β-catenin signaling in hematopoietic stem cells and megakaryocytes
Authors: Shooshtarizadeh, Peiman and Helness, Anne and Vadnais, Charles and Brouwer, Nelleke and Beauchemin, Hugues and Chen, Riyan and Bagci, Halil and Staal, Frank JT and Coté, Jean-Frančois and Möröy, Tarik
Journal: Nature communications (2019): 1270
Authors: Shooshtarizadeh, Peiman and Helness, Anne and Vadnais, Charles and Brouwer, Nelleke and Beauchemin, Hugues and Chen, Riyan and Bagci, Halil and Staal, Frank JT and Coté, Jean-Frančois and Möröy, Tarik
Journal: Nature communications (2019): 1270
A novel dual-well array chip for efficiently trapping single-cell in large isolated micro-well without complicated accessory equipment
Authors: Wang, Chenyu and Liu, Wenwen and Wei, Qingquan and Ren, Lufeng and Tan, Manqing and Yu, Yude
Journal: Biomicrofluidics (2018): 034103
Authors: Wang, Chenyu and Liu, Wenwen and Wei, Qingquan and Ren, Lufeng and Tan, Manqing and Yu, Yude
Journal: Biomicrofluidics (2018): 034103
References
View all 23 references: Citation Explorer
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Journal: Electrophoresis (2003): 200
Model for focal demyelination of the spinal dorsal columns of transgenic MBP-LacZ mice by phototargeted ablation of oligodendrocytes
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Journal: J Neurosci Res (2000): 28
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