FastClick™ 5-FAM Azide
FastClick™ 5-FAM Azide contains both the CAG moiety of FastClick (for assisting click efficiency) and 5-FAM fluorophore (as the fluorescence tag) for developing 5-FAM-based fluorescent probes. 5-FAM is one of the most widely used green fluorophores, in particular, for labeling peptides. It has almost the identical fluorescence spectra to Alexa Fluor 488, a rhodamine 110 analog. FastClick™ reagents have been developed by the scientists of AAT Bioquest for enhancing the yield and reaction speed of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. They contain a copper-chelating ligand that significantly stabilizes the Cu(I) oxidation state and thus accelerates the click reaction. They do not require the use of an external copper-chelator (such as the common THPTA or BTTAA). The high concentration of copper chelators is known to have a detrimental effect on DNA/RNA, thus causing biocompatibility issues. The introduction of a copper-chelating moiety at the reporter molecule allows for a dramatic raise of the effective Cu(I) concentration at the reaction site and thus accelerates the reaction. Under extremely mild conditions the FastClick™ azides and alkynes react much faster in high yield compared to the corresponding conventional CuAAC reactions. Click chemistry was developed by K. Barry Sharpless as a robust and specific method of ligating two molecules together. Two important characteristics make click chemistry attractive for assembling biomolecules. First, click reactions are bio-orthogonal, thus the click chemistry-functionalized biomolecules would not react with the natural biomolecules that lack a clickable functional group. Second, the reactions proceed with ease under mild conditions, such as at room temperature and in aqueous media.
References
View all 9 references: Citation Explorer
Programmed immobilization of living cells using independent click pairs.
Authors: Zhu, Chengyuan and Takemoto, Hiroyasu and Higuchi, Yuriko and Yamashita, Fumiyoshi
Journal: Biochemical and biophysical research communications (2024): 149556
Authors: Zhu, Chengyuan and Takemoto, Hiroyasu and Higuchi, Yuriko and Yamashita, Fumiyoshi
Journal: Biochemical and biophysical research communications (2024): 149556
Manipulating Diastereomeric Bicyclononynes to Sensitively Determine Enzyme Activity and Facilitate Macromolecule Conjugations.
Authors: Huang, Chien-Hui and Hou, Sin-Yu and Severance, Scott and Hwang, Chi-Ching and Fang, Bo-Kai and Gong, Ming-Mao and Yu, Siao-Lei and Weng, Yung-Chieh and Wang, Li-Fang and Dai, Chia-Yen and Wang, Su-Hung and Kuo, Hsing-Tao and Wang, Jeh-Jeng and Wang, Tzu-Pin
Journal: ACS omega (2023): 46073-46090
Authors: Huang, Chien-Hui and Hou, Sin-Yu and Severance, Scott and Hwang, Chi-Ching and Fang, Bo-Kai and Gong, Ming-Mao and Yu, Siao-Lei and Weng, Yung-Chieh and Wang, Li-Fang and Dai, Chia-Yen and Wang, Su-Hung and Kuo, Hsing-Tao and Wang, Jeh-Jeng and Wang, Tzu-Pin
Journal: ACS omega (2023): 46073-46090
The development of potent, competitive CXCR4 antagonists for the prevention of cancer metastasis.
Authors: Hamshaw, Isabel and Cominetti, Marco M D and Lai, Wing-Yee and Searcey, Mark and Mueller, Anja
Journal: Biochemical pharmacology (2023): 115921
Authors: Hamshaw, Isabel and Cominetti, Marco M D and Lai, Wing-Yee and Searcey, Mark and Mueller, Anja
Journal: Biochemical pharmacology (2023): 115921
Concerning the photophysics of fluorophores towards tailored bioimaging compounds: a case study involving S100A9 inflammation markers.
Authors: Steiner, Simon T and Maisuls, Iván and Junker, Anna and Fritz, Günter and Faust, Andreas and Strassert, Cristian A
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2023): 2093-2104
Authors: Steiner, Simon T and Maisuls, Iván and Junker, Anna and Fritz, Günter and Faust, Andreas and Strassert, Cristian A
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2023): 2093-2104
Design and Synthesis of Fluorophore-Tagged Disparlure Enantiomers to Study Pheromone Enantiomer Discrimination in the Pheromone-Binding Proteins from the Gypsy Moth, Lymantria dispar.
Authors: Pinnelli, Govardhana R and Plettner, Erika
Journal: Journal of chemical ecology (2021)
Authors: Pinnelli, Govardhana R and Plettner, Erika
Journal: Journal of chemical ecology (2021)
Page updated on October 28, 2024