FAM-xtra CPG *500A*
Fluorescein CPGs have traditionally been used to add the fluorescein label at the 3'-terminus. FAM-xtra is a new fluorophore that has the identical spectra to FAM, but is much brighter and more photostable than FAM. FAM-xtra CPG is used to add the significantly improved FAM label at the 3'-terminus in complimentary to our FAM-xtra Phosphoramidite that contains no 4,4'-dimethoxytrityl (DMT) group and can be added only once at the 5'-terminus, thereby terminating synthesis. Both FAM-xtra products are single isomers and use an amide linkage which is stable during cleavage and deprotection and does not allow isomer formation. FAM-xtra CPG allows effective blockage of the 3'-terminus from polymerase extension as well as exonuclease digestion. This support can be used in a manner identical to normal protected nucleoside support since it contains the DMT group. It can be deprotected using ammonium hydroxide as required by nucleobases. When using AMA, a small amount of a non-fluorescent impurity might be formed. Alternatively, it may be first deprotected with ammonium hydroxide for 30 minutes at room temperature, followed by the addition of an equal volume of 40% methylamine. The deprotection is completed as required by the nucleobases - e.g. 10 minutes at 65°C or 2 hours at room temperature for standard bases etc.
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
FAM-xtra™ Phosphoramidite | 493 | 517 | 83000 | 0.32 | 0.178 |
References
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Authors: Kochetkova, Olga Yu and Demina, Tatiana S and Antonova, Olga Yu
Journal: Polymers (2022)
Authors: Kochetkova, Olga Yu and Demina, Tatiana S and Antonova, Olga Yu
Journal: Polymers (2022)
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Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2021): 17843-17848
Authors: Kimmel, Blaise R and Mrksich, Milan
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2021): 17843-17848
Physical mapping of repetitive oligonucleotides facilitates the establishment of a genome map-based karyotype to identify chromosomal variations in peanut.
Authors: Fu, Liuyang and Wang, Qian and Li, Lina and Lang, Tao and Guo, Junjia and Wang, Siyu and Sun, Ziqi and Han, Suoyi and Huang, Bingyan and Dong, Wenzhao and Zhang, Xinyou and Du, Pei
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Journal: BMC plant biology (2021): 107
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Journal: Analytica chimica acta (2021): 87-94
Authors: Shen, Ruidi and Zhang, Ji and Huang, Wenxiu and Wu, Shaoyong and Li, Gongke and Zou, Seyin and Ling, Liansheng
Journal: Analytica chimica acta (2021): 87-94
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Journal: Nanomaterials (Basel, Switzerland) (2021)
Authors: Nitu, Florentin R and Savu, Lorand and Muraru, Sorin and Stoian, Ioan and Ionită, Mariana
Journal: Nanomaterials (Basel, Switzerland) (2021)
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