Estradiol-HRP conjugate

Estradiol is an estrogen steroid hormone and the major female sex hormone. It is also called 17 beta-estradiol. Estradiol is involved in the regulation of the estrous and menstrual female reproductive cycles. It is responsible for the development of female secondary sexual characteristics and important in the development and maintenance of female reproductive tissues and pregnancy. It also has important effects in many other tissues including bone, fat, skin, liver, and the brain. Though estradiol levels in males are much lower compared to those in females, estradiol has important roles in males as well. An estradiol test measures the amount of the hormone estradiol in your blood. It is also called an E2 test. An estradiol test has a number of clinically important applications. Estradiol-HRP conjugate is a convenient building block for developing estradiol assays.

Example protocol

AT A GLANCE

Application notes

Estradiol-HRP conjugates have been characterized with our ABTS (#11001), TMB (#11003) and ADHP (#11000) peroxidase substrates. A typical of 1:5,000 to 20,000 dilution were used for ELISA application.
Note The suggested working dilution is provided as a guide only. It is recommended that the users titrates the product for use in their tests using proper positive and negative controls.

Formulation

Reconstitue 1 mg powder in 100 µL ddH₂O to make 10 mg/mL stock solution. The final formulation of the reconstitued conjugate is in 100 mM Sodium Phosphate Buffer, pH 6.0 with 2 mg/mL BSA, 3% trehalose, and 0.01% thimerosal as a stabilizer.

References

View all 50 references: Citation Explorer

Luminescence-Sensing Tb-MOF Nanozyme for the Detection and Degradation of Estrogen Endocrine Disruptors.
Authors: Wang, Li and Chen, Yang
Journal: ACS applied materials & interfaces (2020): 8351-8358

Reusable chemiluminescent fiber optic aptasensor for the determination of 17β-estradiol in water samples.
Authors: Yang, Rong and Liu, Jiayao and Song, Dan and Zhu, Anna and Xu, Wenjuan and Wang, Hongliang and Long, Feng
Journal: Mikrochimica acta (2019): 726

Interventions for emergency contraception.
Authors: Shen, Jie and Che, Yan and Showell, Emily and Chen, Ke and Cheng, Linan
Journal: The Cochrane database of systematic reviews (2019): CD001324

Influence of natural organic matter on horseradish peroxidase-mediated removal of 17α-ethinylestradiol: Role of molecular weight.
Authors: Yang, Yun and Li, Jianhua and Shi, Huanhuan and Zhai, Li and Wang, Xing and Gao, Shixiang
Journal: Journal of hazardous materials (2018): 9-16

Chemiluminescence immunoassays for estradiol and ethinylestradiol based on new biotinylated estrogen derivatives.
Authors: Kanso, Hussein and Inguimbert, Nicolas and Istamboulie, Georges and Barthelmebs, Lise and Calas-Blanchard, Carole and Noguer, Thierry
Journal: Analytical biochemistry (2017): 63-68

Page updated on December 17, 2024

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Physical properties

Molecular weight	N/A
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501