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ER-Lite™ Blue-White DPX *Cell-Permeable*
Product key features
- Selective ER Targeting - Specifically labels the endoplasmic reticulum with minimal off-target staining, outperforming conventional ER dyes like DiOC₆(3).
- High Photostability and Brightness - Features a high extinction coefficient and quantum yield, delivering bright and stable fluorescence ideal for extended imaging sessions.
- Large Stokes Shift and Broad Emission - Emits across 430–640 nm with a large Stokes shift, compatible with standard DAPI or UV long-pass filter sets.
- Environment-Sensitive Fluorescence - Responds to changes in solvent polarity with emission shifts and quantum yield variations, enabling dynamic studies of ER conditions.
- Live-Cell and Two-Photon Imaging Compatible - Cell-permeant and non-cytotoxic at working concentrations; supports two-photon microscopy for high-resolution, deep-tissue imaging.
Product description
ER-Lite™ Blue-White DPX is a highly photostable, environment-sensitive fluorescent probe designed for selective imaging of the endoplasmic reticulum (ER) in live cells. As a member of the Dapoxyl™ (DPX) dye family, it exhibits a large Stokes shift, high extinction coefficient, and exceptional quantum yield, making it an optimal tool for fluorescence microscopy. With an excitation maximum at approximately 374 nm, ER-Lite™ Blue-White DPX emits across a broad spectral range (430–640 nm), facilitating visualization using standard DAPI or UV long-pass optical filters.
This probe demonstrates superior selectivity for the ER, with minimal off-target labeling of mitochondria, unlike conventional ER stains such as DiOC₆(3). Its cell-permeant nature enables efficient intracellular uptake, and it remains non-cytotoxic at low working concentrations. The fluorescence properties of ER-Lite™ Blue-White DPX are highly environment-sensitive, shifting to longer emission wavelengths as solvent polarity increases while exhibiting a concomitant decrease in quantum yield. These characteristics allow for the study of ER organization and dynamics under various physiological conditions.
ER-Lite™ Blue-White DPX is also compatible with two-photon microscopy, expanding its applicability for high-resolution, deep-tissue imaging. While aldehyde fixation partially retains staining patterns, significant fluorescence signal loss should be anticipated. When used according to the optimized staining protocol, ER-Lite™ Blue-White DPX enables high-contrast, high-fidelity visualization of the ER, making it a valuable tool for investigating ER structure, function, and its involvement in cellular homeostasis and stress responses.
This probe demonstrates superior selectivity for the ER, with minimal off-target labeling of mitochondria, unlike conventional ER stains such as DiOC₆(3). Its cell-permeant nature enables efficient intracellular uptake, and it remains non-cytotoxic at low working concentrations. The fluorescence properties of ER-Lite™ Blue-White DPX are highly environment-sensitive, shifting to longer emission wavelengths as solvent polarity increases while exhibiting a concomitant decrease in quantum yield. These characteristics allow for the study of ER organization and dynamics under various physiological conditions.
ER-Lite™ Blue-White DPX is also compatible with two-photon microscopy, expanding its applicability for high-resolution, deep-tissue imaging. While aldehyde fixation partially retains staining patterns, significant fluorescence signal loss should be anticipated. When used according to the optimized staining protocol, ER-Lite™ Blue-White DPX enables high-contrast, high-fidelity visualization of the ER, making it a valuable tool for investigating ER structure, function, and its involvement in cellular homeostasis and stress responses.
Spectrum
Page updated on April 15, 2025
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