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ER-Lite™ Blue-White DPX *Cell-Permeable*
Product key features
- Selective ER Targeting - Specifically labels the endoplasmic reticulum with minimal off-target staining, outperforming conventional ER dyes like DiOC₆(3).
- High Photostability and Brightness - Features a high extinction coefficient and quantum yield, delivering bright and stable fluorescence ideal for extended imaging sessions.
- Large Stokes Shift and Broad Emission - Emits across 430–640 nm with a large Stokes shift, compatible with standard DAPI or UV long-pass filter sets.
- Environment-Sensitive Fluorescence - Responds to changes in solvent polarity with emission shifts and quantum yield variations, enabling dynamic studies of ER conditions.
- Live-Cell and Two-Photon Imaging Compatible - Cell-permeant and non-cytotoxic at working concentrations; supports two-photon microscopy for high-resolution, deep-tissue imaging.
Product description
This probe demonstrates superior selectivity for the ER, with minimal off-target labeling of mitochondria, unlike conventional ER stains such as DiOC₆(3). Its cell-permeant nature enables efficient intracellular uptake, and it remains non-cytotoxic at low working concentrations. The fluorescence properties of ER-Lite™ Blue-White DPX are highly environment-sensitive, shifting to longer emission wavelengths as solvent polarity increases while exhibiting a concomitant decrease in quantum yield. These characteristics allow for the study of ER organization and dynamics under various physiological conditions.
ER-Lite™ Blue-White DPX is also compatible with two-photon microscopy, expanding its applicability for high-resolution, deep-tissue imaging. While aldehyde fixation partially retains staining patterns, significant fluorescence signal loss should be anticipated. When used according to the optimized staining protocol, ER-Lite™ Blue-White DPX enables high-contrast, high-fidelity visualization of the ER, making it a valuable tool for investigating ER structure, function, and its involvement in cellular homeostasis and stress responses.
Example protocol
AT A GLANCE
Prepare cells in growth medium
Incubate cells with ER Lite™ Blue-White DPX working solution at 37 °C for 15 - 30 minutes
Analyze under fluorescence microscope at Ex/Em = 375/550 nm (DAPI- Long pass filter set)
Thaw ER-Lite™ Blue-White DPX at room temperature before starting the experiment.
CELL PREPARATION
For guidelines on cell sample preparation, please visit:
https://www.aatbio.com/resources/guides/cell-sample-preparation.html
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add 200 µL of DMSO (Not provided) into the vial of ER-Lite™ Blue-White DPX and mix well to make 100X ER Tracer™ Red stock solution.
Note: 100 µL of 500X ER-Lite™ Blue-White DPX stock solution is enough for one 96-well plate. Unused 100X ER-Lite™ Blue-White DPX stock solution can be stored at ≤ -20 ºC. Protect from light.
PREPARATION OF WORKING SOLUTION
Add 200 µL of 100X ER-Lite™ Blue-White DPX stock solution into 20 mL of HH buffer, and mix well to make ER-Lite™ Blue-White DPX working solution.
Note: This ER-Lite™ Blue-White DPX working solution is stable for at least 2 hours at room temperature. Protect from light.
SAMPLE EXPERIMENTAL PROTOCOL
- Plate and treat cells as desired.
- Remove cell culture medium. Cells can be washed twice with buffer of your choice.
Add 100 µL/well (96-well plate) or 50 µL/well (384-well plate) of ER-Lite™ Blue-White DPX working solution in the cell plate. Incubate cells with working solution at 37 °C for 15 - 30 minutes, protected from light.
Note: The optimal concentration of the ER probe varies depending on the specific application. The staining conditions may be modified according to the particular cell type and the permeability of the cells or tissues to the probe.
Remove ER-Lite™ Blue-White DPX working solution in each well. Wash cells with physically relevant buffer three times.
- Observe the fluorescence signal in cells using fluorescence microscope with DAPI Long pass filter set (Ex/Em = 375/550 nm).
Spectrum
Safety data sheet