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EDANS C5 maleimide
EDANS is one of the most popular donors for developing FRET-based nucleic acid probes (such as Molecular Beacons) and protease substrates. EDANS is often paired with DABCYL or DABSYL in FRET-based probes. Its fluorescence is environment-sensitive. EDANS C5 maleimide is a thiol-reactive EDANS derivative. It is water-soluble, and has the same spectral properties with EDANS.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) | Correction Factor (260 nm) |
| EDANS C2 maleimide | 336 | 455 | 5900 | - | 0.107 | - |
| XFD488 C5 Maleimide *Same Structure to Alexa Fluor™ 488 C5 Maleimide* | 499 | 520 | 71000 | 0.921 | 0.11 | 0.30 |
| XFD350 C5 Maleimide *Same Structure to Alexa Fluor™ 350 C5 Maleimide* | 343 | 441 | 19000 | - | 0.19 | 0.25 |
| XFD532 C5 Maleimide *Same Structure to Alexa Fluor™ 532 C5 Maleimide* | 534 | 553 | 81000 | 0.611 | 0.09 | 0.24 |
| XFD594 C5 Maleimide *Same Structure to Alexa Fluor™ 594 C5 Maleimide* | 590 | 618 | 90000 | 0.661 | 0.56 | 0.43 |
| XFD405 C5 maleimide | 401 | 421 | 35,000 | - | 0.70 | 0.23 |
| XFD430 C5 maleimide | 432 | 540 | 15,000 | - | 0.28 | - |
| XFD546 C5 maleimide | 561 | 572 | 112000 | 0.791 | 0.12 | 0.21 |
| XFD568 C5 maleimide | 579 | 603 | 91300 | 0.691 | 0.46 | 0.45 |
References
View all 3 references: Citation Explorer
Synthesis and evaluation of fluorogenic triglycerides as lipase assay substrates.
Authors: Andersen, Rokhsana J and Brask, Jesper
Journal: Chemistry and physics of lipids (2016): 72-9
Authors: Andersen, Rokhsana J and Brask, Jesper
Journal: Chemistry and physics of lipids (2016): 72-9
FRET-Protease-Coupled Peptidyl-Prolyl cis-trans Isomerase Assay: New Internally Quenched Fluorogenic Substrates for High-Throughput Screening.
Authors: Caporale, Andrea and Mascanzoni, Fabiola and Farina, Biancamaria and Sturlese, Mattia and Di Sorbo, Gianluigi and Fattorusso, Roberto and Ruvo, Menotti and Doti, Nunzianna
Journal: Journal of biomolecular screening (2016): 701-12
Authors: Caporale, Andrea and Mascanzoni, Fabiola and Farina, Biancamaria and Sturlese, Mattia and Di Sorbo, Gianluigi and Fattorusso, Roberto and Ruvo, Menotti and Doti, Nunzianna
Journal: Journal of biomolecular screening (2016): 701-12
Characterization and inhibition of SARS-coronavirus main protease.
Authors: Liang, Po-Huang
Journal: Current topics in medicinal chemistry (2006): 361-76
Authors: Liang, Po-Huang
Journal: Current topics in medicinal chemistry (2006): 361-76
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