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EDANS C2 maleimide

EDANS C2 maleimide is a thiol-reactive EDANS derivative. It is water-soluble, and has the same spectral properties with EDANS.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of EDANS C2 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM288.725 µL1.444 mL2.887 mL14.436 mL28.873 mL
5 mM57.745 µL288.725 µL577.451 µL2.887 mL5.775 mL
10 mM28.873 µL144.363 µL288.725 µL1.444 mL2.887 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
AMCA C2 Maleimide346434190000.153
DABCYL C2 maleimide---0.516
XFD647 C2 Maleimide *Same Structure to Alexa Fluor™ 647 C2 Maleimide*6506712390000.03
EDANS C5 maleimide33645559000.107

References

View all 54 references: Citation Explorer
The beta4-beta8 Groove Is an ATP-interactive Site in the alpha Crystallin Core Domain of the Small Heat Shock Protein, Human alphaB Crystallin
Authors: Ghosh JG, Houck SA, Doneanu CE, Clark JI.
Journal: J Mol Biol (2006): 364
Recombinant expression and partial characterization of an active soluble histo-aspartic protease from Plasmodium falciparum
Authors: Xiao H, Sinkovits AF, Bryksa BC, Ogawa M, Yada RY.
Journal: Protein Expr Purif (2006): 88
Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus
Authors: Warfield R, Bardelang P, Saunders H, Chan WC, Penfold C, James R, Thomas NR.
Journal: Org Biomol Chem (2006): 3626
A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases
Authors: Weimer S, Oertel K, Fuchsbauer HL.
Journal: Anal Biochem (2006): 110
Characterization and inhibition of SARS-coronavirus main protease
Authors: Liang PH., undefined
Journal: Curr Top Med Chem (2006): 361
Page updated on July 26, 2023

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Physical properties

Molecular weight

346.35

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.107

Extinction coefficient (cm -1 M -1)

5900

Excitation (nm)

336

Emission (nm)

455

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

2149639-01-8
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