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EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]
EDANS is one of the most popular donors for developing FRET-based nucleic acid probes (such as Molecular Beacons) and protease substrates. EDANS is often paired with DABCYL or DABSYL in FRET-based probes. Its fluorescence is environment-sensitive.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 375.488 µL | 1.877 mL | 3.755 mL | 18.774 mL | 37.549 mL |
| 5 mM | 75.098 µL | 375.488 µL | 750.976 µL | 3.755 mL | 7.51 mL |
| 10 mM | 37.549 µL | 187.744 µL | 375.488 µL | 1.877 mL | 3.755 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Correction Factor (280 nm) |
| DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1* | 0.516 |
Citations
View all 1 citations: Citation Explorer
Peptidic “Molecular Beacon” for Collagen
Authors: Yang, Jinyi and Quan, Yifan and Ouyang, Yifu and Tan, Kong Ooi and Weber, Ralph T and Griffin, Robert G and Raines, Ronald T
Journal: Biomacromolecules (2024)
Authors: Yang, Jinyi and Quan, Yifan and Ouyang, Yifu and Tan, Kong Ooi and Weber, Ralph T and Griffin, Robert G and Raines, Ronald T
Journal: Biomacromolecules (2024)
References
View all 54 references: Citation Explorer
The beta4-beta8 Groove Is an ATP-interactive Site in the alpha Crystallin Core Domain of the Small Heat Shock Protein, Human alphaB Crystallin
Authors: Ghosh JG, Houck SA, Doneanu CE, Clark JI.
Journal: J Mol Biol (2006): 364
Authors: Ghosh JG, Houck SA, Doneanu CE, Clark JI.
Journal: J Mol Biol (2006): 364
Recombinant expression and partial characterization of an active soluble histo-aspartic protease from Plasmodium falciparum
Authors: Xiao H, Sinkovits AF, Bryksa BC, Ogawa M, Yada RY.
Journal: Protein Expr Purif (2006): 88
Authors: Xiao H, Sinkovits AF, Bryksa BC, Ogawa M, Yada RY.
Journal: Protein Expr Purif (2006): 88
Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus
Authors: Warfield R, Bardelang P, Saunders H, Chan WC, Penfold C, James R, Thomas NR.
Journal: Org Biomol Chem (2006): 3626
Authors: Warfield R, Bardelang P, Saunders H, Chan WC, Penfold C, James R, Thomas NR.
Journal: Org Biomol Chem (2006): 3626
A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases
Authors: Weimer S, Oertel K, Fuchsbauer HL.
Journal: Anal Biochem (2006): 110
Authors: Weimer S, Oertel K, Fuchsbauer HL.
Journal: Anal Biochem (2006): 110
Characterization and inhibition of SARS-coronavirus main protease
Authors: Liang PH., undefined
Journal: Curr Top Med Chem (2006): 361
Authors: Liang PH., undefined
Journal: Curr Top Med Chem (2006): 361
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