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DNP maleimide
A thiol-reactive building block for developing a probe that can be recognized by anti-DNP antibodies; Also an excellent thiol-reactive FRET quencher paired with Trp or Tyr.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DNP maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 326.552 µL | 1.633 mL | 3.266 mL | 16.328 mL | 32.655 mL |
| 5 mM | 65.31 µL | 326.552 µL | 653.104 µL | 3.266 mL | 6.531 mL |
| 10 mM | 32.655 µL | 163.276 µL | 326.552 µL | 1.633 mL | 3.266 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Citations
View all 1 citations: Citation Explorer
Imaging of extracellular cathepsin S activity by a selective near infrared fluorescence substrate-based probe
Authors: Wartenberg, Mylène and Saidi, Ahlame and Galibert, Mathieu and Joulin-Giet, Alix and Burlaud-Gaillard, Julien and Lecaille, Fabien and Scott, Christopher J and Aucagne, Vincent and Delmas, Agnès F and Lalmanach, Gilles
Journal: Biochimie (2019)
Authors: Wartenberg, Mylène and Saidi, Ahlame and Galibert, Mathieu and Joulin-Giet, Alix and Burlaud-Gaillard, Julien and Lecaille, Fabien and Scott, Christopher J and Aucagne, Vincent and Delmas, Agnès F and Lalmanach, Gilles
Journal: Biochimie (2019)
References
View all 124 references: Citation Explorer
Continuous assay of protein tyrosine phosphatases based on fluorescence resonance energy transfer
Authors: Nishikata, M.; Yoshimura, Y.; Deyama, Y.; Suzuki, K.
Journal: Biochimie (2006): 879-86
Authors: Nishikata, M.; Yoshimura, Y.; Deyama, Y.; Suzuki, K.
Journal: Biochimie (2006): 879-86
High-throughput screening of low molecular weight NS3-NS4A protease inhibitors using a fluorescence resonance energy transfer substrate
Authors: Sudo K, Yamaji K, Kawamura K, Nishijima T, Kojima N, Aibe K, Shimotohno K, Shimizu Y.
Journal: Antivir Chem Chemother (2005): 385
Authors: Sudo K, Yamaji K, Kawamura K, Nishijima T, Kojima N, Aibe K, Shimotohno K, Shimizu Y.
Journal: Antivir Chem Chemother (2005): 385
Recombinant human cathepsin X is a carboxymonopeptidase only: a comparison with cathepsins B and L
Authors: Puzer L, Cotrin SS, Cezari MH, Hirata IY, Juliano MA, Stefe I, Turk D, Turk B, Juliano L, Carmona AK.
Journal: Biol Chem (2005): 1191
Authors: Puzer L, Cotrin SS, Cezari MH, Hirata IY, Juliano MA, Stefe I, Turk D, Turk B, Juliano L, Carmona AK.
Journal: Biol Chem (2005): 1191
Evaluation of an imaging platform during the development of a FRET protease assay
Authors: George J, Teear ML, Norey CG, Burns DD.
Journal: J Biomol Screen (2003): 72
Authors: George J, Teear ML, Norey CG, Burns DD.
Journal: J Biomol Screen (2003): 72
Generation of heterogeneous rabbit anti-DNP antibodies by gene conversion and hypermutation of rearranged VL and VH genes during clonal expansion of B cells in splenic germinal centers
Authors: Sehgal D, Schiaffella E, Anderson AO, Mage RG.
Journal: Eur J Immunol (2000): 3634
Authors: Sehgal D, Schiaffella E, Anderson AO, Mage RG.
Journal: Eur J Immunol (2000): 3634
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