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DiI Dye
1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate; CAS 41085-99-8
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiI and its analogs are most frequently used since they usually exhibit very low cell toxicity. In addition, DiI is widely used for determining lipoproteins such as LDL and HDL.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiI perchlorate [1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate] *CAS 41085-99-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 104.024 µL | 520.118 µL | 1.04 mL | 5.201 mL | 10.402 mL |
| 5 mM | 20.805 µL | 104.024 µL | 208.047 µL | 1.04 mL | 2.08 mL |
| 10 mM | 10.402 µL | 52.012 µL | 104.024 µL | 520.118 µL | 1.04 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| DiD Perchlorate *UltraPure Grade* | 646 | 663 | 260000 |
| DiO perchlorate [3,3-Dioctadecyloxacarbocyanine perchlorate] *CAS#: 34215-57-1* | 483 | 501 | - |
| DiI iodide [1,1-Dioctadecyl-3,3,3,3- tetramethylindocarbocyanine iodide] | 550 | 564 | 148000 |
| DiI triflate [1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine triflate] | 550 | 564 | 148000 |
Citations
View all 51 citations: Citation Explorer
Engineering Central Substitutions in Heptamethine Dyes for Improved Fluorophore Performance
Authors: Guo, Lei and Yang, Meek and Dong, Bin and Lewman, Seth and Van Horn, Alex and Jia, Shang
Journal: JACS Au (2024)
Authors: Guo, Lei and Yang, Meek and Dong, Bin and Lewman, Seth and Van Horn, Alex and Jia, Shang
Journal: JACS Au (2024)
In situ reprogramming of tumor-associated macrophages enhances cancer immunotherapy
Authors: Han, Yanjie and Lu, Haifeng and Gu, Zikuan and Guan, Peixin and Liu, Zhen
Journal: Nano Today (2024): 102420
Authors: Han, Yanjie and Lu, Haifeng and Gu, Zikuan and Guan, Peixin and Liu, Zhen
Journal: Nano Today (2024): 102420
Exosomes Secreted by CSFV-Infected Cells Evade Neutralizing Antibody to Activate Innate Immune Responses and Establish Productive Infection in Recipient Cells
Authors: Bao, Xi and Zhuang, Tenhan and Xu, Yue and Chen, Li and Feng, Lei and Yao, Huochun
Journal: Veterinary Microbiology (2024): 110062
Authors: Bao, Xi and Zhuang, Tenhan and Xu, Yue and Chen, Li and Feng, Lei and Yao, Huochun
Journal: Veterinary Microbiology (2024): 110062
Extracellular vesicles from lung tissue drive bone marrow neutrophil recruitment in inflammation
Authors: Liu, Bowen and Jin, Yuan and Yang, Jingyi and Han, Yue and Shan, Hui and Qiu, Mantang and Zhao, Xuyang and Liu, Anhang and Jin, Yan and Yin, Yuxin
Journal: Journal of Extracellular Vesicles (2022): e12223
Authors: Liu, Bowen and Jin, Yuan and Yang, Jingyi and Han, Yue and Shan, Hui and Qiu, Mantang and Zhao, Xuyang and Liu, Anhang and Jin, Yan and Yin, Yuxin
Journal: Journal of Extracellular Vesicles (2022): e12223
Novel fusion peptides deliver exosomes to modify injectable thermo-sensitive hydrogels for bone regeneration
Authors: Ma, Shiqing and Wu, Jinzhe and Hu, Han and Mu, Yuzhu and Zhang, Lei and Zhao, Yifan and Bian, Xiaowei and Jing, Wei and Wei, Pengfei and Zhao, Bo and others,
Journal: Materials Today Bio (2022): 100195
Authors: Ma, Shiqing and Wu, Jinzhe and Hu, Han and Mu, Yuzhu and Zhang, Lei and Zhao, Yifan and Bian, Xiaowei and Jing, Wei and Wei, Pengfei and Zhao, Bo and others,
Journal: Materials Today Bio (2022): 100195
References
View all 92 references: Citation Explorer
Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Functional neuroanatomy of the rhinophore of Aplysia punctata
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Page updated on August 13, 2024
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