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Dihydrofluorescein diacetate [Fluorescin diacetate] *CAS 35340-49-9*
Dihydrofluorescein diacetate (also called fluorescin diacetate) is hydrolyzed by cellular esterases to dihydrofluorescein (also called fluorescin) and is then oxidized to fluorescein primarily by H2O2. Dihydrofluorescein diacetate might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. Cell-loading studies indicate that dihydrofluorescein achieves higher intracellular concentrations than the other redox sensors such as 2',7'-dichlorodihydrofluorescein and Dihydrorhodamine 123.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Dihydrofluorescein diacetate [Fluorescin diacetate] *CAS 35340-49-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 239.006 µL | 1.195 mL | 2.39 mL | 11.95 mL | 23.901 mL |
| 5 mM | 47.801 µL | 239.006 µL | 478.011 µL | 2.39 mL | 4.78 mL |
| 10 mM | 23.901 µL | 119.503 µL | 239.006 µL | 1.195 mL | 2.39 mL |
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References
View all 11 references: Citation Explorer
Kinetic analysis of fluorescein and dihydrofluorescein effluxes in tumour cells expressing the multidrug resistance protein, MRP1
Authors: Saengkhae C, Loetchutinat C, Garnier-Suillerot A.
Journal: Biochem Pharmacol (2003): 969
Authors: Saengkhae C, Loetchutinat C, Garnier-Suillerot A.
Journal: Biochem Pharmacol (2003): 969
Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes
Authors: Diaz G, Liu S, Isola R, Diana A, Falchi AM.
Journal: Histochem Cell Biol (2003): 319
Authors: Diaz G, Liu S, Isola R, Diana A, Falchi AM.
Journal: Histochem Cell Biol (2003): 319
Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123
Authors: Hempel SL, Buettner GR, O'Malley YQ, Wessels DA, Flaherty DM.
Journal: Free Radic Biol Med (1999): 146
Authors: Hempel SL, Buettner GR, O'Malley YQ, Wessels DA, Flaherty DM.
Journal: Free Radic Biol Med (1999): 146
A rapid diagnostic test for the viability of early cattle and rabbit embryos using diacetyl-fluorescin
Authors: Schilling E, Dopke HH.
Journal: Naturwissenschaften (1978): 658
Authors: Schilling E, Dopke HH.
Journal: Naturwissenschaften (1978): 658
Determination of fluorescin sodium in ophthalmic solutions
Authors: Robertson EJ, Patel JA.
Journal: Am J Hosp Pharm (1968): 598
Authors: Robertson EJ, Patel JA.
Journal: Am J Hosp Pharm (1968): 598
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