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Digoxigenin-11-dUTP *1 mM solution in TE Buffer*
Digoxigenin-11-dUTP can be enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting digoxigenin-labeled DNA/cDNA probes are subsequently detected using digoxigenin-antibodies conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. The 11-atom linker is attached to the C5 position of uridine to minimize the interference of DIG on the substrate incorporation by PCR polymerase. Typically1:2 Digoxigenin-11-dUTP/dTTP ratio for PCR and Nick Translation.
Citations
View all 2 citations: Citation Explorer
LncRNA AL592284. 1 facilitates proliferation and metastasis of cervical cancer cells via miR-30a-5p/Vimentin/EMT axis
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
ATF4-mediated transcriptional regulation protects against $\beta$-cell loss during endoplasmic reticulum stress in a mouse model
Authors: Kitakaze, Keisuke and Oyadomari, Miho and Zhang, Jun and Hamada, Yoshimasa and Takenouchi, Yasuhiro and Tsuboi, Kazuhito and Inagaki, Mai and Tachikawa, Masanori and Fujitani, Yoshio and Okamoto, Yasuo and others,
Journal: Molecular Metabolism (2021): 101338
Authors: Kitakaze, Keisuke and Oyadomari, Miho and Zhang, Jun and Hamada, Yoshimasa and Takenouchi, Yasuhiro and Tsuboi, Kazuhito and Inagaki, Mai and Tachikawa, Masanori and Fujitani, Yoshio and Okamoto, Yasuo and others,
Journal: Molecular Metabolism (2021): 101338
References
View all 9 references: Citation Explorer
A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
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Journal: Biosens Bioelectron (2004): 685
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
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