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DiFMUP
DiFMUP is a fluorinated MUP, which is a commonly used fluorogenic alkaline phosphatase substrate. The reaction product of DiFMUP has excitation/emission maxima of ~358/450 nm, essentially identical to the spectral properties of MUP. Upon phosphatase-induced hydrolysis DiFMUP generates fluorinated 4-methylumbelliferyl fluorophore that has a lower pKa than that of the coumarin fluorophore generated by MUP, making DiFMUP a superior substrate for continuously assaying acid phosphatases at low pH than MUP. DiFMUP is also used for monitoring protein phosphatase activities.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiFMUP to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 342.313 µL | 1.712 mL | 3.423 mL | 17.116 mL | 34.231 mL |
| 5 mM | 68.463 µL | 342.313 µL | 684.627 µL | 3.423 mL | 6.846 mL |
| 10 mM | 34.231 µL | 171.157 µL | 342.313 µL | 1.712 mL | 3.423 mL |
Molarity calculator
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Spectrum
Citations
View all 3 citations: Citation Explorer
PPP2R5D promotes hepatitis C virus infection by binding to viral NS5B and enhancing viral RNA replication
Authors: Anwar, Muhammad Ikram and Li, Ni and Zhou, Qing and Chen, Mingxiao and Hu, Chengguang and Wu, Tao and Chen, Haihang and Li, Yi-Ping and Zhou, Yuanping
Journal: Virology Journal (2022): 1--12
Authors: Anwar, Muhammad Ikram and Li, Ni and Zhou, Qing and Chen, Mingxiao and Hu, Chengguang and Wu, Tao and Chen, Haihang and Li, Yi-Ping and Zhou, Yuanping
Journal: Virology Journal (2022): 1--12
Proteomic analysis of the hepatotoxicity of Microcystis aeruginosa in adult zebrafish (Danio rerio) and its potential mechanisms
Authors: Du, Benben and Liu, Guangfu and Ke, Mingjing and Zhang, Zhenyan and Zheng, Meng and Lu, Tao and Sun, Liwei and Qian, Haifeng
Journal: Environmental Pollution (2019): 113019
Authors: Du, Benben and Liu, Guangfu and Ke, Mingjing and Zhang, Zhenyan and Zheng, Meng and Lu, Tao and Sun, Liwei and Qian, Haifeng
Journal: Environmental Pollution (2019): 113019
Spontaneous and chaperone-assisted metal loading in the active site of protein phosphatase-1
Authors: Van der Hoeven, Gerd and Lemaire, Sarah and Cao, Xinyu and Claes, Zander and Karamanou, Spyridoula and Bollen, Mathieu
Journal: FEBS letters
Authors: Van der Hoeven, Gerd and Lemaire, Sarah and Cao, Xinyu and Claes, Zander and Karamanou, Spyridoula and Bollen, Mathieu
Journal: FEBS letters
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