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Di-8-ANEPPS *CAS#: 157134-53-7*
Di-8-ANEPPS is widely used for monitoring fast membrane potential changes. ANEP dyes belong to the class of the fast-response membrane potential dyes. Their optical response is fast enough to detect transient membrane potential changes in excitable cells where they demonstrate a membrane potential-dependent shift in excitation spectra. This feature allows the measurement of membrane potential changes by excitation ratio. These dyes are weakly fluorescent in aqueous media, and become strongly fluorescent upon binding to lipophilic environments (such as membranes). Di-8-ANEPPS is less susceptible for cellular internalization than other ANEP dyes probably due to its sulfonate group. In general, fast-response probes operate by means of a change in their electronic structure, and consequently their fluorescence properties, in response to a change in the surrounding electric field. Their optical response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells and intact brains. However, the magnitude of their potential-dependent fluorescence change is often small; fast-response probes typically show a 2-10% fluorescence change per 100 mV.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Di-8-ANEPPS *CAS#: 157134-53-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 168.668 µL | 843.341 µL | 1.687 mL | 8.433 mL | 16.867 mL |
| 5 mM | 33.734 µL | 168.668 µL | 337.336 µL | 1.687 mL | 3.373 mL |
| 10 mM | 16.867 µL | 84.334 µL | 168.668 µL | 843.341 µL | 1.687 mL |
Molarity calculator
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| Di-4-ANEPPS *CAS#: 90134-00-2* | 482 | 686 | 31622 |
Citations
View all 4 citations: Citation Explorer
Destructive role of TMAO in T-tubule and excitation-contraction coupling in the adult cardiomyocytes
Authors: Jin, Bu and Ji, Fangfang and Zuo, Anjun and Liu, Huiting and Qi, Lin and He, Yun and Wang, Qingyao and Zhao, Peng
Journal: International Heart Journal (2020): 355--363
Authors: Jin, Bu and Ji, Fangfang and Zuo, Anjun and Liu, Huiting and Qi, Lin and He, Yun and Wang, Qingyao and Zhao, Peng
Journal: International Heart Journal (2020): 355--363
Cholesterol is required for maintaining T-tubule integrity and intercellular connections at intercalated discs in cardiomyocytes
Authors: Zhu, Yanqi and Zhang, Caimei and Chen, Biyi and Chen, Rong and Guo, Ang and Hong, Jiang and Song, Long-Sheng
Journal: Journal of molecular and cellular cardiology (2016): 204--212
Authors: Zhu, Yanqi and Zhang, Caimei and Chen, Biyi and Chen, Rong and Guo, Ang and Hong, Jiang and Song, Long-Sheng
Journal: Journal of molecular and cellular cardiology (2016): 204--212
Preserving transverse tubule function in adult cardiac myocytes in short-term culture.
Authors: Young, Kester EJ and Young, Kester EJ
Journal: Cardiol : 804--812
Authors: Young, Kester EJ and Young, Kester EJ
Journal: Cardiol : 804--812
References
View all 109 references: Citation Explorer
Optical imaging of medullary ventral respiratory network during eupnea and gasping in situ
Authors: Potts JT, Paton JF.
Journal: Eur J Neurosci (2006): 3025
Authors: Potts JT, Paton JF.
Journal: Eur J Neurosci (2006): 3025
Membrane dipole potential as measured by ratiometric 3-hydroxyflavone fluorescence probes: accounting for hydration effects
Authors: M'Baye G, Shynkar VV, Klymchenko AS, Mely Y, Duportail G.
Journal: J Fluoresc (2006): 35
Authors: M'Baye G, Shynkar VV, Klymchenko AS, Mely Y, Duportail G.
Journal: J Fluoresc (2006): 35
Cholesterol effect on the dipole potential of lipid membranes
Authors: Starke-Peterkovic T, Turner N, Vitha MF, Waller MP, Hibbs DE, Clarke RJ.
Journal: Biophys J (2006): 4060
Authors: Starke-Peterkovic T, Turner N, Vitha MF, Waller MP, Hibbs DE, Clarke RJ.
Journal: Biophys J (2006): 4060
Three fluorescent protein voltage sensors exhibit low plasma membrane expression in mammalian cells
Authors: Baker BJ, Lee H, Pieribone VA, Cohen LB, Isacoff EY, Knopfel T, Kosmidis EK.
Journal: J Neurosci Methods. (2006)
Authors: Baker BJ, Lee H, Pieribone VA, Cohen LB, Isacoff EY, Knopfel T, Kosmidis EK.
Journal: J Neurosci Methods. (2006)
Imaging of cardiac movement using ratiometric and nonratiometric optical mapping: effects of ischemia and 2, 3-butaneodione monoxime
Authors: Himel HDt, Knisley SB.
Journal: IEEE Trans Med Imaging (2006): 122
Authors: Himel HDt, Knisley SB.
Journal: IEEE Trans Med Imaging (2006): 122
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