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Di-2-ANEPEQ [JPW 1114]
Di-2-ANEPEQ is used for monitoring fast membrane potential changes. ANEP dyes belong to the class of the fast-response membrane potential dyes. Their optical response is fast enough to detect transient membrane potential changes in excitable cells where they demonstrate a membrane potential-dependent shift in excitation spectra. This feature allows the measurement of membrane potential changes by excitation ratio. These dyes are weakly fluorescent in aqueous media, and become strongly fluorescent upon binding to lipophilic environments (such as membranes). In general, fast-response probes operate by means of a change in their electronic structure, and consequently their fluorescence properties, in response to a change in the surrounding electric field. Their optical response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells and intact brains. However, the magnitude of their potential-dependent fluorescence change is often small; fast-response probes typically show a 2-10% fluorescence change per 100 mV.
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Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Di-2-ANEPEQ [JPW 1114] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 182.023 µL | 910.117 µL | 1.82 mL | 9.101 mL | 18.202 mL |
| 5 mM | 36.405 µL | 182.023 µL | 364.047 µL | 1.82 mL | 3.64 mL |
| 10 mM | 18.202 µL | 91.012 µL | 182.023 µL | 910.117 µL | 1.82 mL |
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Citations
View all 1 citations: Citation Explorer
Cholesterol is required for maintaining T-tubule integrity and intercellular connections at intercalated discs in cardiomyocytes
Authors: Zhu, Yanqi and Zhang, Caimei and Chen, Biyi and Chen, Rong and Guo, Ang and Hong, Jiang and Song, Long-Sheng
Journal: Journal of molecular and cellular cardiology (2016): 204--212
Authors: Zhu, Yanqi and Zhang, Caimei and Chen, Biyi and Chen, Rong and Guo, Ang and Hong, Jiang and Song, Long-Sheng
Journal: Journal of molecular and cellular cardiology (2016): 204--212
References
View all 109 references: Citation Explorer
Optical imaging of medullary ventral respiratory network during eupnea and gasping in situ
Authors: Potts JT, Paton JF.
Journal: Eur J Neurosci (2006): 3025
Authors: Potts JT, Paton JF.
Journal: Eur J Neurosci (2006): 3025
Membrane dipole potential as measured by ratiometric 3-hydroxyflavone fluorescence probes: accounting for hydration effects
Authors: M'Baye G, Shynkar VV, Klymchenko AS, Mely Y, Duportail G.
Journal: J Fluoresc (2006): 35
Authors: M'Baye G, Shynkar VV, Klymchenko AS, Mely Y, Duportail G.
Journal: J Fluoresc (2006): 35
Cholesterol effect on the dipole potential of lipid membranes
Authors: Starke-Peterkovic T, Turner N, Vitha MF, Waller MP, Hibbs DE, Clarke RJ.
Journal: Biophys J (2006): 4060
Authors: Starke-Peterkovic T, Turner N, Vitha MF, Waller MP, Hibbs DE, Clarke RJ.
Journal: Biophys J (2006): 4060
Three fluorescent protein voltage sensors exhibit low plasma membrane expression in mammalian cells
Authors: Baker BJ, Lee H, Pieribone VA, Cohen LB, Isacoff EY, Knopfel T, Kosmidis EK.
Journal: J Neurosci Methods. (2006)
Authors: Baker BJ, Lee H, Pieribone VA, Cohen LB, Isacoff EY, Knopfel T, Kosmidis EK.
Journal: J Neurosci Methods. (2006)
Imaging of cardiac movement using ratiometric and nonratiometric optical mapping: effects of ischemia and 2, 3-butaneodione monoxime
Authors: Himel HDt, Knisley SB.
Journal: IEEE Trans Med Imaging (2006): 122
Authors: Himel HDt, Knisley SB.
Journal: IEEE Trans Med Imaging (2006): 122
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