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DEAC,SE [7-Diethylaminocoumarin-3-carboxylic acid, succinimidyl ester] *CAS 139346-57-9*
The amine-reactive 7-diethylaminocoumarin-3-carboxylic acid, succinimidyl ester can be used to create blue-fluorescent bioconjugates. It has quite strong blue fluorescence. When compared with AMCA conjugates, conjugates of the UV-light-excitable 7-dialkylaminocoumarin fluorophore have much longer-wavelength excitation and slightly longer-wavelength emission spectra (~470 nm). This fluorescent dye is quite hydrophobic, and might be used for labeling live cells.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DEAC,SE [7-Diethylaminocoumarin-3-carboxylic acid, succinimidyl ester] *CAS 139346-57-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 279.057 µL | 1.395 mL | 2.791 mL | 13.953 mL | 27.906 mL |
| 5 mM | 55.811 µL | 279.057 µL | 558.114 µL | 2.791 mL | 5.581 mL |
| 10 mM | 27.906 µL | 139.528 µL | 279.057 µL | 1.395 mL | 2.791 mL |
Molarity calculator
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| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Correction Factor (280 nm) |
| SunRed™ SE | 592 | 609 | 0.366 |
| DEAC-dUTP *1 mM in TE Buffer (pH 7.5)* | 411 | 472 | 0.12 |
| CDCFDA, SE [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester] *Mixed isomers* | 505 | 526 | - |
References
View all 4 references: Citation Explorer
Fluorescent agonists for the Torpedo nicotinic acetylcholine receptor
Authors: Krieger F, Mourot A, Araoz R, Kotzyba-Hibert F, Molgo J, Bamberg E, Goeldner M.
Journal: Chembiochem (2008): 1146
Authors: Krieger F, Mourot A, Araoz R, Kotzyba-Hibert F, Molgo J, Bamberg E, Goeldner M.
Journal: Chembiochem (2008): 1146
A series of related nucleotide analogues that aids optimization of fluorescence signals in probing the mechanism of P-loop ATPases, such as actomyosin
Authors: Webb MR, Reid GP, Munasinghe VR, Corrie JE.
Journal: Biochemistry (2004): 14463
Authors: Webb MR, Reid GP, Munasinghe VR, Corrie JE.
Journal: Biochemistry (2004): 14463
Fluorescent coumarin-labeled nucleotides to measure ADP release from actomyosin
Authors: Webb MR, Corrie JE.
Journal: Biophys J (2001): 1562
Authors: Webb MR, Corrie JE.
Journal: Biophys J (2001): 1562
Glutamate 779, an intramembrane carboxyl, is essential for monovalent cation binding by the Na,K-ATPase
Authors: Arguello JM, Kaplan JH.
Journal: J Biol Chem (1994): 6892
Authors: Arguello JM, Kaplan JH.
Journal: J Biol Chem (1994): 6892
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