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ddTTP [2',3'-Dideoxythymidine-5'-triphosphate] *10 mM in ddH2O*
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase. It was developed by Frederick Sanger and colleagues in 1977. Although the newer NGS technologies are becoming common in clinical research labs due to their higher throughput capabilities and lower costs per sample, Sanger sequencing with 99.99% accuracy is still the “gold standard” for clinical research sequencing. dd-TTP is one of the four critical ddNTP components for performing Sanger sequencing.
References
View all 10 references: Citation Explorer
Strong positional preference in the interaction of LNA oligonucleotides with DNA polymerase and proofreading exonuclease activities: implications for genotyping assays.
Authors: Di Giusto, Daniel A and King, Garry C
Journal: Nucleic acids research (2004): e32
Authors: Di Giusto, Daniel A and King, Garry C
Journal: Nucleic acids research (2004): e32
Detection and genotyping of human papillomavirus DNA in cervical cancer tissues with fluorescence polarization.
Authors: Gao, Yan-E and Zhang, Ju and Wu, Jing and Chen, Zhong-Can and Yan, Xiao-Jun
Journal: Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica (2003): 1029-34
Authors: Gao, Yan-E and Zhang, Ju and Wu, Jing and Chen, Zhong-Can and Yan, Xiao-Jun
Journal: Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica (2003): 1029-34
Dye structure affects Taq DNA polymerase terminator selectivity.
Authors: Brandis, J W
Journal: Nucleic acids research (1999): 1912-8
Authors: Brandis, J W
Journal: Nucleic acids research (1999): 1912-8
Synergistic inhibition of HIV-1 reverse transcriptase by combinations of chain-terminating nucleotides.
Authors: Villahermosa, M L and Martinez-Irujo, J J and Cabodevilla, F and Santiago, E
Journal: Biochemistry (1997): 13223-31
Authors: Villahermosa, M L and Martinez-Irujo, J J and Cabodevilla, F and Santiago, E
Journal: Biochemistry (1997): 13223-31
Characterization of the p68/p58 heterodimer of human immunodeficiency virus type 2 reverse transcriptase.
Authors: Fan, N and Rank, K B and Poppe, S M and Tarpley, W G and Sharma, S K
Journal: Biochemistry (1996): 1911-7
Authors: Fan, N and Rank, K B and Poppe, S M and Tarpley, W G and Sharma, S K
Journal: Biochemistry (1996): 1911-7
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