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ddGTP [2',3'-Dideoxyguanosine-5'-triphosphate] *10 mM in ddH2O*
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase. It was developed by Frederick Sanger and colleagues in 1977. Although the newer NGS technologies are becoming common in clinical research labs due to their higher throughput capabilities and lower costs per sample, Sanger sequencing with 99.99% accuracy is still the “gold standard” for clinical research sequencing. dd-GTP is one of the four critical ddNTP components for performing Sanger sequencing.
References
View all 4 references: Citation Explorer
Detection and genotyping of human papillomavirus DNA in cervical cancer tissues with fluorescence polarization.
Authors: Gao, Yan-E and Zhang, Ju and Wu, Jing and Chen, Zhong-Can and Yan, Xiao-Jun
Journal: Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica (2003): 1029-34
Authors: Gao, Yan-E and Zhang, Ju and Wu, Jing and Chen, Zhong-Can and Yan, Xiao-Jun
Journal: Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica (2003): 1029-34
Microcapillary reactors using solid-phase DNA sequencing for direct sample introduction into slab gels.
Authors: Xu, Y and Bruch, R C and Soper, S A
Journal: BioTechniques (2000): 904-8, 910, 912
Authors: Xu, Y and Bruch, R C and Soper, S A
Journal: BioTechniques (2000): 904-8, 910, 912
Isolation and characterization of a dideoxyguanosine triphosphate-resistant mutant of human immunodeficiency virus reverse transcriptase.
Authors: Prasad, V R and Lowy, I and de los Santos, T and Chiang, L and Goff, S P
Journal: Proceedings of the National Academy of Sciences of the United States of America (1991): 11363-7
Authors: Prasad, V R and Lowy, I and de los Santos, T and Chiang, L and Goff, S P
Journal: Proceedings of the National Academy of Sciences of the United States of America (1991): 11363-7
Herpes simplex virus type 1 and human DNA polymerase interactions with 2'-deoxyguanosine 5'-triphosphate analogues. Kinetics of incorporation into DNA and induction of inhibition.
Authors: Reardon, J E
Journal: The Journal of biological chemistry (1989): 19039-44
Authors: Reardon, J E
Journal: The Journal of biological chemistry (1989): 19039-44
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