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ddCTP [2',3'-Dideoxycytidine-5'-triphosphate] *10 mM in ddH2O*
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase. It was developed by Frederick Sanger and colleagues in 1977. Although the newer NGS technologies are becoming common in clinical research labs due to their higher throughput capabilities and lower costs per sample, Sanger sequencing with 99.99% accuracy is still the “gold standard” for clinical research sequencing. dd-CTP is one of the four critical ddNTP components for performing Sanger sequencing.
References
View all 5 references: Citation Explorer
Involvement of DNA polymerase beta in DNA replication and mutagenic consequences.
Authors: Servant, Laurence and Bieth, Anne and Hayakawa, Hiroshi and Cazaux, Christophe and Hoffmann, Jean-Sébastien
Journal: Journal of molecular biology (2002): 1039-47
Authors: Servant, Laurence and Bieth, Anne and Hayakawa, Hiroshi and Cazaux, Christophe and Hoffmann, Jean-Sébastien
Journal: Journal of molecular biology (2002): 1039-47
Fluorescence-based DHPLC for allelic quantification by single-nucleotide primer extension.
Authors: Kosaki, K and Yoshihashi, H and Ohashi, Y and Kosaki, R and Suzuki, T and Matsuo, N
Journal: Journal of biochemical and biophysical methods (2001): 111-9
Authors: Kosaki, K and Yoshihashi, H and Ohashi, Y and Kosaki, R and Suzuki, T and Matsuo, N
Journal: Journal of biochemical and biophysical methods (2001): 111-9
Denaturation fingerprinting: two related mutation detection methods especially advantageous for high G + C regions.
Authors: Liu, Q and Weinshenker, B G and Wingerchuk, D M and Sommer, S S
Journal: BioTechniques (1998): 140-7
Authors: Liu, Q and Weinshenker, B G and Wingerchuk, D M and Sommer, S S
Journal: BioTechniques (1998): 140-7
Synergistic inhibition of HIV-1 reverse transcriptase by combinations of chain-terminating nucleotides.
Authors: Villahermosa, M L and Martinez-Irujo, J J and Cabodevilla, F and Santiago, E
Journal: Biochemistry (1997): 13223-31
Authors: Villahermosa, M L and Martinez-Irujo, J J and Cabodevilla, F and Santiago, E
Journal: Biochemistry (1997): 13223-31
A fidelity assay using "dideoxy" DNA sequencing: a measurement of sequence dependence and frequency of forming 5-bromouracil X guanine base mispairs.
Authors: Lasken, R S and Goodman, M F
Journal: Proceedings of the National Academy of Sciences of the United States of America (1985): 1301-5
Authors: Lasken, R S and Goodman, M F
Journal: Proceedings of the National Academy of Sciences of the United States of America (1985): 1301-5
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