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ddATP [2',3'-Dideoxyadenosine-5'-triphosphate] *10 mM in ddH2O*
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase. It was developed by Frederick Sanger and colleagues in 1977. Although the newer NGS technologies are becoming common in clinical research labs due to their higher throughput capabilities and lower costs per sample, Sanger sequencing with 99.99% accuracy is still the “gold standard” for clinical research sequencing. dd-ATP is one of the four critical ddNTP components for performing Sanger sequencing.
References
View all 4 references: Citation Explorer
Denaturation fingerprinting: two related mutation detection methods especially advantageous for high G + C regions.
Authors: Liu, Q and Weinshenker, B G and Wingerchuk, D M and Sommer, S S
Journal: BioTechniques (1998): 140-7
Authors: Liu, Q and Weinshenker, B G and Wingerchuk, D M and Sommer, S S
Journal: BioTechniques (1998): 140-7
Slow rate of phosphodiester bond formation accounts for the strong bias that Taq DNA polymerase shows against 2',3'-dideoxynucleotide terminators.
Authors: Brandis, J W and Edwards, S G and Johnson, K A
Journal: Biochemistry (1996): 2189-200
Authors: Brandis, J W and Edwards, S G and Johnson, K A
Journal: Biochemistry (1996): 2189-200
Recombinant human hepatitis B virus reverse transcriptase is active in the absence of the nucleocapsid or the viral replication origin, DR1.
Authors: Seifer, M and Standring, D N
Journal: Journal of virology (1993): 4513-20
Authors: Seifer, M and Standring, D N
Journal: Journal of virology (1993): 4513-20
The enzymatic termination of polydeoxynucleotides by 2',3'-dideoxyadenosine triphosphate.
Authors: Toji, L and Cohen, S S
Journal: Proceedings of the National Academy of Sciences of the United States of America (1969): 871-7
Authors: Toji, L and Cohen, S S
Journal: Proceedings of the National Academy of Sciences of the United States of America (1969): 871-7
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